Invitrogen

GNAO1 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated. View Details

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Datasheet

Protocols

Questions & Answers

Tech Support

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GNAO1 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry-Immunofluorescence analysis of GNAO1 was performed in DIV9 rat E18 primary hippocampal neuron cells fixed in 4% paraformaldehyde at RT for 15 min. Green: GNAO1 Polyclonal Antibody (Product # PA5-30044) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody. Blue: Fluoroshield with DAPI.

GNAO1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry (Paraffin) analysis of GNAO1 was performed in paraffin-embedded mouse pancreas tissue using GNAO1 Polyclonal Antibody (Product # PA5-30044) at a dilution of 1:500.

GNAO1 Antibody in Immunohistochemistry (Frozen) (IHC (F)) — Immunohistochemistry (Frozen) analysis of GNAO1 was performed in frozen-sectioned mouse cerebellum tissue using GNAO1 Polyclonal Antibody (Product # PA5-30044) at a dilution of 1:250 (Green). Red: NF-H, stained by NF-H antibody diluted at 1:500. Blue: Fluoroshield with DAPI.

GNAO1 Antibody in Western Blot (WB) — Western blot was performed using Anti-GNAO1 Polyclonal Antibody (Product # PA5-30044) and a 40kDa band corresponding to GNAO1 was observed in Mouse Brain and not in Mouse Lung, Mouse Kidney and Mouse Skeletal Muscle which are reported negative for GNAO1 expression. Tissue extracts (30 µg lysate) of Mouse Brain (Lane 1), Mouse Lung (Lane 2), Mouse Kidney (Lane 3) and Mouse Skeletal Muscle (Lane 4) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence Goat Anti-Rabbit IgG Secondary Antibody, HRP conjugate (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

GNAO1 Antibody in Immunoprecipitation (IP) — Immunoprecipitation and Western blot of GNAO1 protein from HeLa whole cell extracts using 5 µg of GNAO1 antibody (Product # PA5-30044).

GNAO1 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across tissues owing to their inherent genetic constitution. Relative expression of GNAO1 was observed in Mouse Brain in comparison to Mouse Lung, Mouse Kidney and Mouse Skeletal Muscle using GNAO1 Polyclonal Antibody product (Product # PA5-30044) in western blot. . {RE}

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:20,000

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunohistochemistry (Frozen) (IHC (F))

1:100-1:1,000

Immunocytochemistry (ICC/IF)

1:100-1:1,000

Immunoprecipitation (IP)

1:100-1:500

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 104 and 338 of Human GNAO1

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 1% BSA, 20% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2547518

Product Specific Information

Recommended positive controls: U87-MG, SK-N-SH, IMR32, SK-N-AS, mouse brain, mouse lung, rat brain, rat lung.

Predicted reactivity: Mouse (97%), Rat (98%), Xenopus laevis (98%), Chicken (98%), Rhesus Monkey (100%), Bovine (98%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Heterotrimeric G proteins are membrane bound GTPases that are linked to 7-TM receptors. Each G protein contains an alpha-, beta- and gamma-subunit and is bound to GDP in the 'off' state. Ligand-receptor binding results in detachment of the G protein, switching it to an 'on' state and permitting Galpha activation of second messenger signalling cascades. There are several types of Galpha proteins; Galphas and Galphai bind directly to adenylyl cyclase and stimulate or inhibit its activity, leading to an increase or decrease in cAMP levels respectively.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: DKFZp686O0962; G alpha o; GNAO 1; Go alpha; GO2-q chimeric G-protein; GTP-binding protein alpha o; GTP-binding protein alpha-o subunit; guanine nucleotide binding protein (G protein), alpha activating activity polypeptide O; guanine nucleotide binding protein, alpha O polypeptide 1; Guanine nucleotide-binding protein G(o) subunit alpha; guanine nucleotide-binding regulatory protein 2

Gene Aliases: alphaO; AW050213; EIEE17; G-ALPHA-o; Galphao; Gna0; GNAO; GNAO1; HLA-DQB1; RATBPGTPC

UniProt ID: (Human) P09471, (Rat) P59215, (Mouse) P18872

Entrez Gene ID: (Human) 2775, (Rat) 50664, (Mouse) 14681

Molecular Function: heterotrimeric G-protein