|Tested species reactivity||Human, Mouse, Non-human primate, Rat|
|Published species reactivity||Human|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Purified recombinant fragment of human GOT2 expressed in E. Coli.|
|Contains||0.03% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||1/200 - 1/1000|
|Western Blot (WB)||1/500 - 1/2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
MA5-15595 targets GOT2 in IF and WB applications and shows reactivity with Human, mouse, Non-human primate, and Rat samples.
The MA5-15595 immunogen is purified recombinant fragment of human GOT2 expressed in E. Coli.
MA5-15595 detects GOT2 which has a predicted molecular weight of approximately 47kDa.
Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
APOBEC3G inhibits microRNA-mediated repression of translation by interfering with the interaction between Argonaute-2 and MOV10.
MA5-15595 was used in western blot to study the role of the AGO2/MOV10 interaction in the mechanism by which APOBEC3G blocks miRNA-mediated translational repression
|Liu C,Zhang X,Huang F,Yang B,Li J,Liu B,Luo H,Zhang P,Zhang H||The Journal of biological chemistry (287:29373)||2012|