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Immunohistochemical staining of paraformaldehyde-fixed paraffin-embedded human tonsil sections at 200x (A) and 400x (B) magnification with DAPI counterstain. Primary antibody used was a GR-1 monoclonal antibody (clone RB6-8C5) at a 1:500 dilution, followed by secondary antibody was a 488 fluorescent conjugated Goat anti-rat at a 1:500 dilution and Normal Goat Serum (blocking)
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rat / IgG2b|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1 ug/1 million cells|
|Immunohistochemistry (Paraffin, Frozen) (IHC (P, F))||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA1-70099 detects Gr-1 in mouse samples.
MA1-70099 has been successfully used in flow cytometry, Western blot and immunohistochemistry procedures.
The MA1-70099 immunogen is Gr-1.
Store at 4°C. For long term storage, aliquot and store at -20°C, avoiding freeze/thaw cycles.
Granulocytes are a category of white blood cells characterised by the presence of granules in their cytoplasm. They are also called polymorphonuclear leukocytes (PMN or PML) because of the varying shapes of the nucleus, which is usually lobed into three segments. In common parlance, the term polymorphonuclear leukocyte often refers specifically to neutrophil granulocytes, the most abundant of the granulocytes. Granulocytes or PMN are released from the bone marrow by the regulatory complement proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
epididymis luminal protein 75; epididymis secretory sperm binding protein Li 122m; glutathione reductase; glutathione reductase 1; glutathione reductase, mitochondrial; glutathione S-reductase; GR; GRase; PML; PMN; polymorphonuclear leukocytes
AI325518; D8Ertd238e; GLUR; Gr-1; Gr1; GRD1; GSR; HEL-75; HEL-S-122m