Invitrogen

GRB2 Monoclonal Antibody (11-B11)

Product Image Gallery

GRB2 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of GRB2 in MCF-7 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with GRB2 monoclonal antibody (Product # MA5-34887), followed by DAPI (blue).

GRB2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of GRB2 in paraffin-embedded human colon cancer tissue. Samples were incubated with GRB2 monoclonal antibody (Product # MA5-34887), and followed by hematoxylin.

GRB2 Antibody in Western Blot (WB) — Western blot analysis of GRB2 in 293 cell lysate. Samples were incubated with GRB2 monoclonal antibody (Product # MA5-34887), at a dilution of 1:1000.

Product Details

MA5-34887

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:200	-
Immunocytochemistry (ICC/IF)	1:50-1:200	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Mouse / IgG2b
Class	Monoclonal
Type	Antibody
Clone	11-B11
Immunogen	Recombinant protein within Human GRB2 aa 21-217 View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	2 mg/mL
Purification	Protein G
Storage buffer	PBS, pH 7.4, with 50% glycerol, 0.2% BSA
Contains	0.05% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles, store in dark
RRID	AB_2848794

Product Specific Information

Positive Control: 293, MCF-7, HepG2, rat brain tissue, human colon cancer tissue,

Target Information

GRB 2 protein contains one SH2 domain flanked on both sides by an SH3 domain. In response to EGF, GRB 2 binds, through its SH3 domains, to the guanine nucleotide exchange factor, Sos. The GRB 2-Sos complex then binds the EGF receptor. These interactions play a critical role in the activation of the p21 ras signaling pathway.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: abundant SRC homology; Adapter protein GRB2; ASH protein; epidermal growth factor receptor-binding protein GRB2; Growth factor receptor-bound protein 2; growth factor receptor-bound protein 3; HT027; OTTHUMP00000166097; OTTHUMP00000166098; Protein Ash; SH2/SH3 adapter GRB2

Gene Aliases: ASH; EGFRBP-GRB2; GRB2; Grb3-3; MST084; MSTP084; NCKAP2

UniProt ID: (Human) P62993

Entrez Gene ID: (Human) 2885

Molecular Function: scaffold/adaptor protein