Invitrogen

GRB2 Recombinant Rabbit Monoclonal Antibody (ARC0430)

View all (17) GRB2 antibodies

Questions & Answers

Questions & Answers

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GRB2 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemical analysis of GRB2 performed on HeLa cells using a GRB2 monoclonal antibody (Product # MA5-35238) at a dilution of 1:100. An HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was applied at a dilution of 1:10,000.

GRB2 Antibody in Immunohistochemistry (IHC) — Immunohistochemical analysis of GRB2 performed on paraffin embedded human lung cancer using a GRB2 monoclonal antibody (Product # MA5-35238) at a dilution of 1:100. An HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was applied at a dilution of 1:10,000.

GRB2 Antibody in Immunohistochemistry (IHC) — Immunohistochemical analysis of GRB2 performed on paraffin embedded mouse spleen using a GRB2 monoclonal antibody (Product # MA5-35238) at a dilution of 1:100. An HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was applied at a dilution of 1:10,000.

GRB2 Antibody in Immunohistochemistry (IHC) — Immunohistochemical analysis of GRB2 performed on paraffin embedded rat spleen using a GRB2 monoclonal antibody (Product # MA5-35238) at a dilution of 1:100. An HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was applied at a dilution of 1:10,000.

GRB2 Antibody in Western Blot (WB) — Western Blot analysis of GRB2 performed on various cell lines using a GRB2 monoclonal antibody (Product # MA5-35238) at a dilution of 1:1,000. An HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was applied at a dilution of 1:10,000.

Product Details

MA5-35238

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	-
Immunohistochemistry (IHC)	1:50-1:200	-
Immunocytochemistry (ICC/IF)	1:100	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Recombinant Monoclonal
Type	Antibody
Clone	ARC0430
Immunogen	A synthesized peptide derived from human GRB2
Conjugate	Unconjugated
Form	Liquid
Concentration	0.2 mg/mL
Purification	Protein A
Storage buffer	PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains	0.02% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2849141

Target Information

GRB 2 protein contains one SH2 domain flanked on both sides by an SH3 domain. In response to EGF, GRB 2 binds, through its SH3 domains, to the guanine nucleotide exchange factor, Sos. The GRB 2-Sos complex then binds the EGF receptor. These interactions play a critical role in the activation of the p21 ras signaling pathway.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: abundant SRC homology; Adapter protein GRB2; ASH protein; epidermal growth factor receptor-binding protein GRB2; Growth factor receptor-bound protein 2; growth factor receptor-bound protein 3; HT027; OTTHUMP00000166097; OTTHUMP00000166098; Protein Ash; SH2/SH3 adapter GRB2

Gene Aliases: AA408164; ASH; EGFRBP-GRB2; GRB2; Grb3-3; MST084; MSTP084; NCKAP2

UniProt ID: (Human) P62993, (Mouse) Q60631, (Rat) P62994

Entrez Gene ID: (Human) 2885, (Mouse) 14784, (Rat) 81504

Molecular Function: scaffold/adaptor protein

MAPK cascade epidermal growth factor receptor signaling pathway Ras protein signal transduction cell-cell signaling axon guidance insulin receptor signaling pathway fibroblast growth factor receptor signaling pathway positive regulation of signal transduction regulation of phosphatidylinositol 3-kinase signaling viral process positive regulation of actin filament polymerization T cell costimulation receptor internalization phosphatidylinositol-3-phosphate biosynthetic process Fc-epsilon receptor signaling pathway Fc-gamma receptor signaling pathway involved in phagocytosis ERBB2 signaling pathway negative regulation of epidermal growth factor receptor signaling pathway signal transduction in response to DNA damage regulation of MAPK cascade positive regulation of GTPase activity phosphatidylinositol phosphorylation phosphatidylinositol-mediated signaling anatomical structure formation involved in morphogenesis leukocyte migration branching involved in labyrinthine layer morphogenesis cellular response to ionizing radiation positive regulation of reactive oxygen species metabolic process aging cell differentiation protein heterooligomerization

Process(es)

SH3/SH2 adaptor activity Ras guanyl-nucleotide exchange factor activity epidermal growth factor receptor binding neurotrophin TRKA receptor binding protein binding 1-phosphatidylinositol-3-kinase activity SH3 domain binding protein kinase binding protein phosphatase binding identical protein binding insulin receptor substrate binding poly(A) RNA binding ephrin receptor binding phosphatidylinositol-4,5-bisphosphate 3-kinase activity phosphotyrosine binding protein domain specific binding phosphoprotein binding