Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
1:500-1:2,000 | - |
Immunohistochemistry (IHC) |
1:50-1:200 | - |
Immunocytochemistry (ICC/IF) |
1:100 | - |
Product Specifications | |
---|---|
Species Reactivity |
Human, Mouse, Rat |
Host/Isotype |
Rabbit / IgG |
Class |
Recombinant Monoclonal |
Type |
Antibody |
Clone |
ARC0430 |
Immunogen |
A synthesized peptide derived from human GRB2 |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
0.2 mg/mL |
Purification |
Protein A |
Storage buffer |
PBS, pH 7.3, with 50% glycerol, 0.05% BSA |
Contains |
0.02% sodium azide |
Storage conditions |
-20° C, Avoid Freeze/Thaw Cycles |
RRID |
AB_2849141 |
GRB 2 protein contains one SH2 domain flanked on both sides by an SH3 domain. In response to EGF, GRB 2 binds, through its SH3 domains, to the guanine nucleotide exchange factor, Sos. The GRB 2-Sos complex then binds the EGF receptor. These interactions play a critical role in the activation of the p21 ras signaling pathway.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: abundant SRC homology; Adapter protein GRB2; ASH protein; epidermal growth factor receptor-binding protein GRB2; Growth factor receptor-bound protein 2; growth factor receptor-bound protein 3; HT027; OTTHUMP00000166097; OTTHUMP00000166098; Protein Ash; SH2/SH3 adapter GRB2
Gene Aliases: AA408164; ASH; EGFRBP-GRB2; GRB2; Grb3-3; MST084; MSTP084; NCKAP2
UniProt ID: (Human) P62993, (Mouse) Q60631, (Rat) P62994
Entrez Gene ID: (Human) 2885, (Mouse) 14784, (Rat) 81504
Molecular Function:
scaffold/adaptor protein
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