|Tested species reactivity||Human|
|Published species reactivity||Mouse|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Recombinant human GRIP1 protein|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunoprecipitation (IP)||2 µg/ml|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|ChIP assay (ChIP)||See 1 publications below|
MA5-11553 targets GRIP1 in IP and WB applications and shows reactivity with Human samples.
The MA5-11553 immunogen is recombinant human GRIP1 protein.
The steroid hormones, thyroid hormone, vitamin D, and retinoic acid function by activating specific "nuclear receptor proteins" found inside of target cells. The activated receptors bind to and activate specific genes, and the products of these activated genes change cellular activities in specific ways, thus constituting the biological response to the hormone. After binding to target genes, the activated nuclear receptors require the assistance of proteins called "transcriptional co-activators" in order to activate the target genes. GRIP1 (Glucocorticoid Receptor Interacting Protein 1) is a novel protein that serves as a transcriptional co-activator for steroid and nuclear hormone receptors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
TGF-beta-activated Smad3 represses MEF2-dependent transcription in myogenic differentiation.
MA5-11553 was used in ChIP assay to study the mechanism for the inhibitory effect of transforming growth factor beta on myogenesis
|Liu D,Kang JS,Derynck R||The EMBO journal (23:1557)||2004|