MA1-721 detects G-protein-associated rhodopsin kinase 1a/b (GRK1a/b) from human and bovine tissues. This antibody does not cross-react with other G protein-coupled kinases.
MA1-721 has been successfully used in Western blot, immunofluorescence, and immunoprecipitation experiments. By Western blot, this antibody detects an ~60 kDa protein representing GRK 1a/b. MA1-721 detects both in vitro expressed splice variants by Western blot but was not able to detect significant amounts of GRK1b from human retina. Immunofluorescence staining of GRK1a/b in bovine retina with MA1-721 results in intense staining primarily in cone outer segments, but also in rod outer segments. Weak staining is observed in somata and synaptic terminals of cones and inner segments of rods.
The MA1-721 immunogen is full length human GRK1a. This antibody recognizes an N-terminal epitope of human GRK1 which is conserved in both GRK1a and GRK1b.
The sensation of sight is the result of a cascade of events starting with the interaction of photoactivated rhodopsin with a protein called transducin. Rhodopsin kinase is a G-protein-coupled Ser/Thr kinase, also known as GRK1, which is a key element in the regulation of this cascade. Following phosphorylation by GRK1, arrestin is recruited to phospho-rhodopsin quenching its phototransductive activity by preventing further interaction with transducin. By breaking the cycle of phototransduction, GRK1 plays an important role in the restoration of the system for subsequent visual events.
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Protein Aliases: G protein-coupled receptor kinase 1; G-protein receptor kinase 1; GRK 1a/b; GRK1a; rhodopsin kinase; Rhodopsin kinase GRK1; RK
Gene Aliases: GPRK1; GRK1; RHOK; RK
UniProt ID: (Human) Q15835