Invitrogen

GRK1 Monoclonal Antibody (G8)

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

10 Published Figures

21 References

View all (15) GRK1 antibodies

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Datasheet

Protocols

Questions & Answers

Tech Support

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GRK1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of Rhodopsin Kinase 1a was done on 70% confluent log phase MCF-7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Rhodopsin Kinase 1a (G8) Mouse Monoclonal Antibody (Product # MA1-720) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d is a merged image showing Cytoplasmic localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.

GRK1 Antibody in Immunohistochemistry (IHC) — Immunolocalization of GRK1a in bovine retina using Product # MA1-720.

GRK1 Antibody in Western Blot (WB) — Published figure using GRK1 monoclonal antibody (Product # MA1-720) in Western Blot

GRK1 Antibody — Figure 2 Protein expression in wild-type, Arr1 +/- , GRK1 +/- , and GRK1 +/- Arr1 +/- rods. (A) Representative immublot analysis of arrestin and GRK1 expression in retinas of wild-type and heterozygous knockout mice. The blot was probed with polyclonal anti-arrestin, monoclonal anti-rhododopsin kinase 1a, and monoclonal anti- beta tubulin antibodies. Two different dilutions of the same samples were run on the same blot. (B) Levels of arrestin1 and GRK1 expression in heterozygous knockout retinas normalized to the levels in wild-type retinas. Integrated fluorescence intensity values were used for all analyses. Variations in sample loading were corrected for by normalizing the intensity value of each protein band to the intensity value of beta -tubulin in the same lane. Open circles and triangles show the results from two different dilutions. Normalized ratios of arrestin1 and GRK1 expression compared to wild-type values for Arr1 +/- were 0.34 +- 0.06 and 0.87 +- 0.07 (mean +- SEM, n = 5); 1.14 +- 0.10 and 0.32 +- 0.03 for GRK1 +/- (n = 4); and 0.54 +- 0.09 and 0.34 +- 0.03 for GRK1 +/- Arr1 +/- (n = 5). (C) Levels of PDE alphabeta , PDE gamma and RGS9-1 expression in heterozygous retinas normalized to the levels in wild-type retinas. Normalized ratios of PDE alphabeta , PDE gamma and RGS9-1 compared to wild-type values for Arr1 +/- were 0.97 +- 0.03, 1.06 +- 0.05, and 1.05 +- 0.07 (n = 4); 0.86 +- 0.03, 0.99 +- 0.04, and 1.05 +- 0.07 for GRK1 +/- (n = 4); 0.89 +- 0.05, 0.99 +- {KO}

Product Details

MA1-720

Applications	Tested Dilution	Publications
Western Blot (WB)	1 µg/mL	View 15 publications 15 publications
Immunohistochemistry (IHC)	Assay-dependent	View 4 publications 4 publications
Immunocytochemistry (ICC/IF)	2-3 µg/mL	View 2 publications 2 publications
Immunoprecipitation (IP)	Assay-dependent	-

Product Specifications
Species Reactivity	Bovine, Chicken, Human, Mouse, Rat
Published species	Bovine, Dog, Human, Mouse, Rat
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	G8
Immunogen	Full length human GRK1. View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Protein G
Contains	0.05% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2115613

Product Specific Information

MA1-720 detects G-protein-associated rhodopsin kinase 1a (GRK1a) from human, mouse, rat, bovine and chicken tissues.

MA1-720 has been successfully used in Western blot, immunofluorescence and immunoprecipitation procedures. By Western blot, this antibody detects an ~60 kDa protein representing GRK1a from bovine rod outer segments. Immunofluorescence staining of GRK1a in bovine retina with MA1-720 results in intense staining primarily in cone outer segments, but also in rod outer segments. Weak staining is observed in somata and synaptic terminals of cones and inner segments of rods.

The MA1-720 immunogen is full length human GRK1. This antibody recognizes an epitope in the C-terminal region of GRK1a.

Target Information

The sensation of sight is the result of a cascade of events starting with the interaction of photoactivated rhodopsin with a protein called transducin. Rhodopsin kinase is a G-protein-coupled Ser/Thr kinase, also known as GRK1, which is a key element in the regulation of this cascade. Following phosphorylation by GRK1, arrestin is recruited to phospho-rhodopsin quenching its phototransductive activity by preventing further interaction with transducin. By breaking the cycle of phototransduction, GRK1 plays an important role in the restoration of the system for subsequent visual events.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

ARL13B, a Joubert Syndrome-Associated Protein, Is Critical for Retinogenesis and Elaboration of Mouse Photoreceptor Outer Segments.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Dilan TL,Moye AR,Salido EM,Saravanan T,Kolandaivelu S,Goldberg AFX,Ramamurthy V

Published figure using GRK1 monoclonal antibody (Product # MA1-720) in Western Blot

Wed Feb 20 00:00:00 EST 2019

The age-regulating protein klotho is vital to sustain retinal function.

Investigative ophthalmology & visual science

Reish NJ,Maltare A,McKeown AS,Laszczyk AM,Kraft TW,Gross AK,King GD

MA1-720 was used in western blot to study the expression of klotho in murine retina and its role in maintaining retinal function

Fri Oct 11 00:00:00 EDT 2013

The age-regulating protein klotho is vital to sustain retinal function.

Investigative ophthalmology & visual science

Reish NJ,Maltare A,McKeown AS,Laszczyk AM,Kraft TW,Gross AK,King GD

MA1-720 was used in western blot to study the expression of klotho in murine retina and its role in maintaining retinal function

Fri Oct 11 00:00:00 EDT 2013

Modulation of mouse rod response decay by rhodopsin kinase and recoverin.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Chen CK,Woodruff ML,Chen FS,Chen Y,Cilluffo MC,Tranchina D,Fain GL

MA1-720 was used in western blot to study the role of rhodopsin kinase and recoverin in murine rod response decay

Wed Nov 07 00:00:00 EST 2012

Tubby is required for trafficking G protein-coupled receptors to neuronal cilia.

Cilia

Sun X,Haley J,Bulgakov OV,Cai X,McGinnis J,Li T

Published figure using GRK1 monoclonal antibody (Product # MA1-720) in Immunofluorescence

Thu Nov 01 00:00:00 EDT 2012

Replacing the rod with the cone transducin subunit decreases sensitivity and accelerates response decay.

The Journal of physiology

Chen CK,Woodruff ML,Chen FS,Shim H,Cilluffo MC,Fain GL

MA1-720 was used in western blot to investigate the mechanism why cone vision is less sensitive than rod vision

Wed Sep 01 00:00:00 EDT 2010

Control of rhodopsin's active lifetime by arrestin-1 expression in mammalian rods.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Gross OP,Burns ME

MA1-720 was used in Western Blotting to reveal the contribution of Arr1 binding to the overall time course of rhodopsin deactivation.

Wed Mar 03 00:00:00 EST 2010

Background light produces a recoverin-dependent modulation of activated-rhodopsin lifetime in mouse rods.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Chen CK,Woodruff ML,Chen FS,Chen D,Fain GL

Published figure using GRK1 monoclonal antibody (Product # MA1-720) in Immunohistochemistry

Wed Jan 27 00:00:00 EST 2010

Background light produces a recoverin-dependent modulation of activated-rhodopsin lifetime in mouse rods.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Chen CK,Woodruff ML,Chen FS,Chen D,Fain GL

Published figure using GRK1 monoclonal antibody (Product # MA1-720) in Immunohistochemistry

Wed Jan 27 00:00:00 EST 2010

CREB1/ATF1 activation in photoreceptor degeneration and protection.

Investigative ophthalmology & visual science

Beltran WA,Allore HG,Johnson E,Towle V,Tao W,Acland GM,Aguirre GD,Zeiss CJ

MA1-720 was used in western blot to study the expression levels of p-CREB1, CREB1, and ATF1 in photoreceptor disease and protection.

Sun Nov 01 00:00:00 EDT 2009

Arrestin competition influences the kinetics and variability of the single-photon responses of mammalian rod photoreceptors.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Doan T,Azevedo AW,Hurley JB,Rieke F

MA1-720 was used in western blot to study the effect of GRK1 and arrestin 1 on the kinetics and variability of single-photon responses of a rod.

Wed Sep 23 00:00:00 EDT 2009

High levels of retinal membrane docosahexaenoic acid increase susceptibility to stress-induced degeneration.

Journal of lipid research

Tanito M,Brush RS,Elliott MH,Wicker LD,Henry KR,Anderson RE

MA1-720 was used in western blot to study the effect of retinal membrane docosahexaenoic acid on stress-induced retinal degeneration in mice.

Fri May 01 00:00:00 EDT 2009

Overexpression of rhodopsin alters the structure and photoresponse of rod photoreceptors.

Biophysical journal

Wen XH,Shen L,Brush RS,Michaud N,Al-Ubaidi MR,Gurevich VV,Hamm HE,Lem J,Dibenedetto E,Anderson RE,Makino CL

Published figure using GRK1 monoclonal antibody (Product # MA1-720) in Western Blot

Sun Feb 01 00:00:00 EST 2009

Flupirtine attenuates sodium nitroprusside-induced damage to retinal photoreceptors, in situ.

Brain research bulletin

Fawcett RJ,Osborne NN

MA1-720 was used in immunohistochemistry to study the mechanism by which flupirtine attenuates sodium nitroprusside-induced damage to retinal photoreceptors

Thu Jul 12 00:00:00 EDT 2007

Differentiation of embryonic stem cells to retinal cells in vitro.

Methods in molecular biology (Clifton, N.J.)

Zhao X,Liu J,Ahmad I

MA1-720 was used in immunocytochemistry to develop a method for the in vitro differentiation of embryonic stem cells to retinal cells

Tue Aug 22 00:00:00 EDT 2006

Transducin activation state controls its light-dependent translocation in rod photoreceptors.

The Journal of biological chemistry

Kerov V,Chen D,Moussaif M,Chen YJ,Chen CK,Artemyev NO

MA1-720 was used in western blot to investigate the role of the GTP-hydrolysis on Gtalpha for light-dependent translocation in rod photoreceptors

Fri Dec 09 00:00:00 EST 2005

Functional characterization of mouse RDH11 as a retinol dehydrogenase involved in dark adaptation in vivo.

The Journal of biological chemistry

Kasus-Jacobi A,Ou J,Birch DG,Locke KG,Shelton JM,Richardson JA,Murphy AJ,Valenzuela DM,Yancopoulos GD,Edwards AO

MA1-720 was used in western blot to demonstrate the effect of mRDH11 disruption on retinoid metabolism in photoreceptor inner segments .

Fri May 27 00:00:00 EDT 2005

The bovine iris-ciliary epithelium expresses components of rod phototransduction.

Neuroscience letters

Ghosh S,Salvador-Silva M,Coca-Prados M

MA1-720 was used in western blot to investigate the level of phototransduction proteins in bovine iris-ciliary epithelium

Wed Nov 03 00:00:00 EST 2004

Leber congenital amaurosis linked to AIPL1: a mouse model reveals destabilization of cGMP phosphodiesterase.

Proceedings of the National Academy of Sciences of the United States of America

Ramamurthy V,Niemi GA,Reh TA,Hurley JB

MA1-720 was used in western blot to investigate the essential role of photoreceptor-specific gene Aryl hydrocarbon interacting protein like 1 (AIPL1) in the retina of a mouse Leber congenital amaurosis model.

Tue Sep 21 00:00:00 EDT 2004

Cone deactivation kinetics and GRK1/GRK7 expression in enhanced S cone syndrome caused by mutations in NR2E3.

Investigative ophthalmology & visual science

Cideciyan AV,Jacobson SG,Gupta N,Osawa S,Locke KG,Weiss ER,Wright AF,Birch DG,Milam AH

MA1-720 was used in immunocytochemistry to study the visual abnormalities in patients with NR2E3 mutations

Sat Mar 01 00:00:00 EST 2003

Phosphorylation of photolyzed rhodopsin is calcium-insensitive in retina permeabilized by alpha-toxin.

Proceedings of the National Academy of Sciences of the United States of America

Otto-Bruc AE,Fariss RN,Van Hooser JP,Palczewski K

MA1-720 was used in western blot to investigate the influence of calcium on rhodopsin phosphorylation in retina

Tue Dec 08 00:00:00 EST 1998

Bioinformatics

Protein Aliases: G protein-coupled receptor kinase 1; G protein-coupled receptpr kinase 1; G-protein receptor kinase 1; GRK 1a/b; GRK1a; rhodopsin kinase; Rhodopsin kinase GRK1; RK

Gene Aliases: GPRK1; GRK1; RHOK; RK

UniProt ID: (Human) Q15835, (Bovine) P28327, (Rat) Q63651

Entrez Gene ID: (Human) 6011, (Chicken) 395431, (Bovine) 281457, (Mouse) 24013, (Rat) 81760

Molecular Function: non-receptor serine/threonine protein kinase