MA1-2013 detects the beta andrenergic receptor kinase 1 in human, mouse and rat samples.
MA1-2013 has been successfully used in Western blot, immunocytochemistry, immunofluoresence, immunoprecipitation, and ELISA procedures. By Western blot this antibody detects a ~30 kDa protein representing the beta andrenergeic receptor kinase 1 in human HeLa cells. In immunocytochemistry procedures MA1-2013 recognizes the beta andrenergic receptor kinase 1 in HeLa, Hek-293 and 3T3 cells. Immunofluoresence in HeLa cells yeilds predominantely cytoplasmic membrane associated staining with some staining in the ruffles.
The MA1-2013 immunogen is recombinant beta adrenergic receptor kinase 1.
G protein-coupled receptor kinases (GRKs) are important regulators of G protein-coupled receptors(GPCRs). GRK2 (83 kDa), one of six members of this family that have been identified, is ubiquitously expressed in mammals. After binding to their ligand and interacting with heterotrimeric G proteins, GPCRs (e.g., a2-adrenergic receptor) are phosphorylated by GRKs. Internalization of the GPCRs, regulated by beta-arrestin-1, leads to activation of the Ras - Raf - ERK1 and 2 signaling pathway. GRK2 activity is tightly controlled by different mechanisms including phosphorylation by kinases such as PKC, Src and ERK1 and 2, as well as interaction with various proteins. ERK phosphorylates and thus inactivates GRK2 on serine 670 in a negative feedback mechanism.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: adrenergic beta receptor kinase 1; adrenergic receptor kinase, beta 1; adrenergic, beta, receptor kinase 1; Beta-adrenergic receptor kinase 1; beta-adrenergic receptor kinase 1 beta ARK1; beta-AR kinase-1; Beta-ARK-1; EC 18.104.22.168; G-protein coupled receptor kinase 2; G-protein-coupled receptor kinase 2; grk 2
Gene Aliases: Adrbk-1; ADRBK1; BARK; Bark-1; BARK1; beta ARK; BETA-ARK1; betaARK1; GRK-2; GRK2