|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues 550-600 of human GRP78|
|Storage buffer||PBS with 0.05% BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||5 µg/ml|
|Western Blot (WB)||1-3 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is expected to cross-react with rat, chimpanzee, canine, macaque monkey, zebrafish, and xenopus laevis based on sequence homology.
Grp78, is a 78 kDa glucose-regulated protein, also known as BiP or immunoglobulin heavy chain binding protein, is a stress-response protein which is induced by agents or conditions that adversely affect endoplasmic reticulum (ER) function. This protein is essential for the proper glycosylation, folding and assembly of many membrane bound and secreted proteins. Grp78 is critical for maintenance of cell homeostasis and the prevention of apoptosis. Grp78 protein levels have been shown to be a reliable biomarker of hypoglycermia as well as serving a neuroprotective function in neurons exposed to glutamate and oxidative stress. Grp78 levels are reduced in the brains of Alzheimer's Disease patients and decreased expression of Grp78 is found associated with missense mutations in the human presenilin-1 (PS1) gene. The induction of the Grp78 protein has been associated with the development of drug-resistance to antitumor drugs.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.