|Tested species reactivity||Chicken, Human, Mouse, Sheep, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide made to an internal portion of the human protein (within residues 250-300).|
|Purification||Antigen affinity chromatography|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:150|
|Western Blot (WB)||0.5 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with zebrafish, c.
elegans, primate, bovine, and drosophila based on 100% sequence homology.
Suggested positive control: HeLa whole cell extract.
GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER). Because so many ER proteins interact transiently with GRP78, it may play a key role in monitoring protein transport through the cell. BiP is also overexpressed in many cancers and has been hypothesized to actively regulate multiple malignant phenotypes including cell growth, migration, and invasion.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.