Immunofluorescent analysis of GRP78/HSPA5 showing staining in the cytoplasm of A431 cells. A431 cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a GRP78/HSPA5 polyclonal antibody (Product # PA5-29705) diluted at 1:500. Blue: Hoechst 33342 staining.
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 340 and 617 of Human Grp78|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 20% glycerol|
|Contains||0.025% Proclin 300|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:1000-1:10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-29705 targets Grp78 in IHC (P), ICC, IF, and WB applications and shows reactivity with Human and mouse samples.
The PA5-29705 immunogen is recombinant fragment corresponding to a region within amino acids 340 and 617 of Human Grp78.
When Chinese hamster K12 cells are starved of glucose, the synthesis of several proteins, called glucose-regulated proteins (GRPs), is markedly increased. Hendershot et al. (1994) [PubMed 8020977] pointed out that one of these, GRP78 (HSPA5), also referred to as 'immunoglobulin heavy chain-binding protein' (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER). Because so many ER proteins interact transiently with GRP78, it may play a key role in monitoring protein transport through the cell.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.