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Immunofluorescent analysis of GRP78/HSPA5 showing staining in the endoplasmic reticulum of HeLa cells. HeLa cells were fixed in ice-cold MeOH for 5 min and stained using a GRP78/HSPA5 polyclonal antibody (Product # PA5-34941) diluted at 1:500. Blue: Hoechst 33342 staining.
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to a region within amino acids 594 and 655 of Hspa5|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:5000-1:20,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-34941 targets GRP78/HSPA5 in IHC (P) and WB applications and shows reactivity with Human, Mouse, and Rat samples.
The PA5-34941 immunogen is synthetic peptide corresponding to a region within amino acids 594 and 655 of Hspa5.
Probably plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
78 kDa glucose-regulated protein; BIP; endoplasmic reticulum lumenal Ca 2+-binding protein grp78; endoplasmic reticulum lumenal Ca(2+)-binding protein grp78; epididymis secretory sperm binding protein Li 89n; glucose regulated protein, 78 kDa; glucose-regulated protein, 78kDa; GRP 78; GRP-78; heat shock 70 kDa protein 5; heat shock 70kD protein 5; heat shock 70kD protein 5 (glucose-regulated protein, 78kD); heat shock 70kDa protein 5 (glucose-regulated protein); heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa); immunoglobulin heavy chain-binding protein; MIF2; steroidogenesis-activator polypeptide; XAP-1 antigen
AL022860; AU019543; baffled; BIP; D2Wsu141e; D2Wsu17e; GRP78; HEL-S-89n; Hsce70; HSPA5; mBiP; MIF2; SEZ-7; Sez7