Immunofluorescent analysis of GRP78/HSPA5 showing staining in the endoplasmic reticulum of HeLa cells. HeLa cells were fixed in ice-cold MeOH for 5 min and stained using a GRP78/HSPA5 polyclonal antibody (Product # PA5-34941) diluted at 1:500. Blue: Hoechst 33342 staining.
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to a region within amino acids 594 and 655 of Hspa5|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:5000-1:20,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-34941 targets GRP78/HSPA5 in IHC (P) and WB applications and shows reactivity with Human, Mouse, and Rat samples.
The PA5-34941 immunogen is synthetic peptide corresponding to a region within amino acids 594 and 655 of Hspa5.
Probably plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate.
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