|Tested species reactivity||Bovine, Dog, Chicken, Horse, Guinea pig, Hamster, Human, Mouse, Non-human primate, Sheep, Pig, Rabbit, Rat, Xenopus|
|Published species reactivity||Human, Mouse|
|Host / Isotype||Rat / IgG2a|
|Immunogen||Purified glucose regulated protein 94 (grp94) from chicken oviducts|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1-2 µg/ml|
|Immunoprecipitation (IP)||10 µg/ml|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-14589 targets Glucose-Regulated Protein 94 in IHC (P), IP, and WB applications and shows reactivity with Bovine, Canine, Chicken, Equine, Guinea Pig, Hamster, Human, mouse, Non-human primate, Ovine, Porcine, Rabbit, Rat, and Xenopus laevis samples.
The MA5-14589 immunogen is purified glucose regulated protein 94 (grp94) from chicken oviducts.
Grp's (Glucose-Regulated Proteins) are a class of proteins synthesized in cells in response to glucose starvation. Glucose-regulated protein 94 (grp94) or tumor rejection antigen (gp96) shows a high degree of sequence homology with the heat shock protein 90 (hsp90). Grp94 has been briefly studied as a prognostic factor in breast cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Glycoprotein 96 perpetuates the persistent inflammation of rheumatoid arthritis.
MA5-14589 was used in ELISA, flow cytometry, and immunohistochemistry to study the role of Toll-like receptors in the mechanism by which endigenous gp-96 maintains the inflammation associated with rheumatoid arthritis
|Huang QQ,Koessler RE,Birkett R,Dorfleutner A,Perlman H,Haines GK,Stehlik C,Nicchitta CV,Pope RM||Arthritis and rheumatism (64:3638)||2012|
HSP60 is transported through the secretory pathway of 3-MCA-induced fibrosarcoma tumour cells and undergoes N-glycosylation.
MA5-14589 was used in western blot to study the secretory pathway transport and N-glycosylation of HSP60 in fibrosarcoma tumor cells
|Hayoun D,Kapp T,Edri-Brami M,Ventura T,Cohen M,Avidan A,Lichtenstein RG||The FEBS journal (279:2083)||2012|
Significances of pH and temperature on the production of heat-shock protein glycoprotein 96 by MethA tumor cell suspension culture in stirred-tank bioreactors.
MA5-14589 was used in western blot to study the effects of temperature and pH on a stirred tank bioreactor system developed to produce heat shock glycoprotein from Meth A tumor cells
|Tang YJ,Li HM,Hamel JF||Bioprocess and biosystems engineering (32:267)||2009|
Suspension culture process of MethA tumor cell for the production of heat-shock protein glycoprotein 96: process optimization in spinner flasks.
MA5-14589 was used in western blot to develop an optimised culture process for the production of heat-shock protein glycoprotein 96 by MethA tumor cells
|Tang YJ,Li HM,Hamel JF||Biotechnology progress (23:1363)||2007|
Induction of heat shock protein gp96 by immune cytokines.
MA5-14589 was used in western blot to study the induction of heat shock protein gp96 by immune cytokines
|Chen YG,Ashok BT,Liu X,Garikapaty VP,Mittelman A,Tiwari RK||Cell stress and chaperones (8:242)||2004|
Molecular chaperones as a common set of proteins that regulate the invasion phenotype of head and neck cancer.
MA5-14589 was used in immunohistochemistry to study the role of molecular chaperones in regulating the invasion phenotype of head and neck cancer
|Chiu CC,Lin CY,Lee LY,Chen YJ,Lu YC,Wang HM,Liao CT,Chang JT,Cheng AJ||Clinical cancer research : an official journal of the American Association for Cancer Research (17:4629)||2011|