|Tested species reactivity||Bovine, Dog, Chicken, Horse, Guinea pig, Hamster, Human, Mouse, Non-human primate, Sheep, Pig, Rabbit, Rat, Xenopus|
|Published species reactivity||Mouse, Human|
|Host / Isotype||Rat / IgG2a|
|Immunogen||Purified glucose regulated protein 94 (grp94) from chicken oviducts|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1-2 µg/ml|
|Immunoprecipitation (IP)||10 µg/ml|
|Western Blot (WB)||1-2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-14592 targets Glucose-Regulated Protein 94 in IHC (P), IP, and WB applications and shows reactivity with Bovine, Canine, Chicken, Equine, Guinea Pig, Hamster, Human, mouse, Non-human primate, Ovine, Porcine, Rabbit, Rat, and Xenopus laevis samples.
The MA5-14592 immunogen is purified glucose regulated protein 94 (grp94) from chicken oviducts.
Grp's (Glucose-Regulated Proteins) are a class of proteins synthesized in cells in response to glucose starvation. Glucose-regulated protein 94 (grp94) or tumor rejection antigen (gp96) shows a high degree of sequence homology with the heat shock protein 90 (hsp90). Grp94 has been briefly studied as a prognostic factor in breast cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
HSP60 is transported through the secretory pathway of 3-MCA-induced fibrosarcoma tumour cells and undergoes N-glycosylation.
MA5-14592 was used in western blot to study the secretory pathway transport and N-glycosylation of HSP60 in fibrosarcoma tumor cells
|Hayoun D,Kapp T,Edri-Brami M,Ventura T,Cohen M,Avidan A,Lichtenstein RG||The FEBS journal (279:2083)||2012|
Significances of pH and temperature on the production of heat-shock protein glycoprotein 96 by MethA tumor cell suspension culture in stirred-tank bioreactors.
MA5-14592 was used in western blot to study the effects of temperature and pH on a stirred tank bioreactor system developed to produce heat shock glycoprotein from Meth A tumor cells
|Tang YJ,Li HM,Hamel JF||Bioprocess and biosystems engineering (32:267)||2009|
Induction of heat shock protein gp96 by immune cytokines.
MA5-14592 was used in western blot to study the induction of heat shock protein gp96 by immune cytokines
|Chen YG,Ashok BT,Liu X,Garikapaty VP,Mittelman A,Tiwari RK||Cell stress and chaperones (8:242)||2004|
Molecular chaperones as a common set of proteins that regulate the invasion phenotype of head and neck cancer.
MA5-14592 was used in immunohistochemistry to study the role of molecular chaperones in regulating the invasion phenotype of head and neck cancer
|Chiu CC,Lin CY,Lee LY,Chen YJ,Lu YC,Wang HM,Liao CT,Chang JT,Cheng AJ||Clinical cancer research : an official journal of the American Association for Cancer Research (17:4629)||2011|