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Invitrogen
GRSF1 Recombinant Rabbit Monoclonal Antibody (7H16L16)
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FIGURE: 1 / 3
GRSF1 Antibody (703097) in WB
Western blot analysis was performed on whole cell extracts (30 µg lysate) of HeLa (Lane 1), MCF-7 (Lane 2), HEK-293 (Lane 3), Hep G2 (Lane 4), U-2 OS (Lane 5), A549 (Lane 6), SH-SY5Y (Lane 7) and A431 (Lane 8). The blot was probed with Anti-GRSF1 Recombinant Rabbit Monoclonal Antibody (Product # 703097, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 1:5000 dilution). A ~52 kDa band corresponding to GRSF1 was observed across the ce... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
703097
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Monoclonal
Type
Antibody
Clone
7H16L16
Immunogen
Protein corresponding to human GRSF1 [aa142-aa245]
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.2-7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
This antibody is predicted to react with Monkey, Bovine, Pig, Mouse.
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
Target Information
G-rich sequence factor 1 (GRSF1) is a regulator of post-transcriptional mitochondrial gene expression. It required for assembly of the mitochondrial ribosome and for recruitment of mRNS and IncRNA. GRSF1 binds RNAs containing the 14 base G-rich element. It preferentially binds RNAs transcribed from three contiguous genes on the light strand of mtDNA, the ND6 mRNA, and the long non-coding RNAs for MT-CYB and MT-NDS - each of which contains multiple consensus binding sequences.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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