Immunofluorescent analysis of GS1 showing staining in the cytoplasm and nucleolus of HepG2 cells. HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a GS1 polyclonal antibody (Product # PA5-30269) diluted at 1:500. Blue: Hoechst 33343 staining.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein fragment corresponding to a region within amino acids 1 and 179 of Human GS1|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-30269 targets GS1 in WB, ICC, and IHC (P) applications and shows reactivity with Human samples.
The PA5-30269 immunogen is recombinant protein fragment corresponding to a region within amino acids 1 and 179 of Human GS1.
This gene encodes a member of the haloacid dehalogenase-like (HAD) hydrolase superfamily. The encoded protein has no known biological function. This gene has a pseudogene on chromosome 1. Multiple alternatively spliced transcript variants encoding different isoforms have been identified.
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