|Tested species reactivity||Mouse, Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant protein fragment corresponding to a region within amino acids 100 and 327 of Human GSK3 beta|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:1000-1:10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-29265 targets GSK3 beta in IHC (P) and WB applications and shows reactivity with Human and mouse samples.
The PA5-29265 immunogen is recombinant protein fragment corresponding to a region within amino acids 100 and 327 of Human GSK3 beta.
Glycogen synthase kinase-3 (GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase (see GYS1, MIM 138570). Two isoforms, alpha (GSK3A; MIM 606784) and beta, show a high degree of amino acid homology (Stambolic and Woodgett, 1994 [PubMed 7980435]). GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation (Plyte et al., 1992 [PubMed 1333807]).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Carbohydrate metabolism Other polysaccharide metabolism Glycogen metabolism Protein metabolism and modification Protein modification Protein phosphorylation Signal transduction Cell surface receptor mediated signal transduction Other receptor mediated signaling pathway Intracellular signaling cascade Developmental processes Embryogenesis Segment specification Ectoderm development Neurogenesis Mesoderm development Cell cycle Mitosis Cell proliferation and differentiation