Invitrogen

GSS Recombinant Rabbit Monoclonal Antibody (JB95-33)

Product Image Gallery

GSS Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of Glutathione Synthetase in LOVO cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Glutathione Synthetase monoclonal antibody (Product # MA5-34698), followed by DAPI (blue).

GSS Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Glutathione Synthetase in paraffin-embedded human colon cancer tissue. Samples were incubated with Glutathione Synthetase monoclonal antibody (Product # MA5-34698), and followed by hematoxylin.

GSS Antibody in Western Blot (WB) — Western blot analysis of Glutathione Synthetase in Lane 1: SiHa, Lane 2: Mouse colon, Lane 3: Rat liver. Samples were incubated with Glutathione Synthetase monoclonal antibody (Product # MA5-34698), at a dilution of 1:500.

GSS Antibody in Flow Cytometry (Flow) — Flow cytometry of Glutathione Synthetase in HepG2 cells (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Samples were incubated with Glutathione Synthetase monoclonal antibody (Product # MA5-34698) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti rabbit IgG.

Product Details

MA5-34698

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:200	-
Immunocytochemistry (ICC/IF)	1:50-1:200	-
Flow Cytometry (Flow)	1:50-1:100	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Rabbit / IgG
Class	Recombinant Monoclonal
Type	Antibody
Clone	JB95-33
Immunogen	Recombinant protein within Human Glutathione Synthetase aa 301-420 View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Protein A
Storage buffer	TBS, pH 7.4, with 40% glycerol, 0.05% BSA
Contains	0.05% sodium azide
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles, store in dark
RRID	AB_2848606

Product Specific Information

Positive Control: SiHa, mouse colon tissue, rat liver tissue lysate, rat epididymis tissue, human tonsil tissue, human colon cancer tissue, human kidney tissue, HepG2, LOVO.

Target Information

Glutathione is important for a variety of biological functions, including protection of cells from oxidative damage by free radicals, detoxification of xenobiotics, and membrane transport. The protein encoded by this gene functions as a homodimer to catalyze the second step of glutathione biosynthesis, which is the ATP-dependent conversion of gamma-L-glutamyl-L-cysteine to glutathione. Defects in this gene are a cause of glutathione synthetase deficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.