Invitrogen

GSS Recombinant Rabbit Monoclonal Antibody (JB95-33)

View all (25) GSS antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

GSS Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of Glutathione Synthetase in LOVO cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with Glutathione Synthetase monoclonal antibody (Product # MA5-34698), followed by DAPI (blue).

GSS Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Glutathione Synthetase in paraffin-embedded human colon cancer tissue. Samples were incubated with Glutathione Synthetase monoclonal antibody (Product # MA5-34698), and followed by hematoxylin.

GSS Antibody in Western Blot (WB) — Western blot analysis of Glutathione Synthetase in Lane 1: SiHa, Lane 2: Mouse colon, Lane 3: Rat liver. Samples were incubated with Glutathione Synthetase monoclonal antibody (Product # MA5-34698), at a dilution of 1:500.

GSS Antibody in Flow Cytometry (Flow) — Flow cytometry of Glutathione Synthetase in HepG2 cells (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Samples were incubated with Glutathione Synthetase monoclonal antibody (Product # MA5-34698) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti rabbit IgG.

Product Details

MA5-34698

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

Immunocytochemistry (ICC/IF)

1:50-1:200

Flow Cytometry (Flow)

1:50-1:100

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

JB95-33

Immunogen

Recombinant protein within Human Glutathione Synthetase aa 301-420

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

TBS, pH 7.4, with 40% glycerol, 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles, store in dark

RRID

AB_2848606

Product Specific Information

Positive Control: SiHa, mouse colon tissue, rat liver tissue lysate, rat epididymis tissue, human tonsil tissue, human colon cancer tissue, human kidney tissue, HepG2, LOVO.

Target Information

Glutathione is important for a variety of biological functions, including protection of cells from oxidative damage by free radicals, detoxification of xenobiotics, and membrane transport. The protein encoded by this gene functions as a homodimer to catalyze the second step of glutathione biosynthesis, which is the ATP-dependent conversion of gamma-L-glutamyl-L-cysteine to glutathione. Defects in this gene are a cause of glutathione synthetase deficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Glutathione synthase; Glutathione synthetase; GSH S; GSH synthetase; GSH-S; GSHB; MGC14098; OTTHUMP00000030711

Gene Aliases: GSHS; GSS; HEL-S-64p; HEL-S-88n

UniProt ID: (Human) P48637

Entrez Gene ID: (Human) 2937

Molecular Function: ligase

Function(s)

cellular amino acid metabolic process glutathione biosynthetic process response to oxidative stress nervous system development aging response to xenobiotic stimulus response to nutrient levels response to tumor necrosis factor response to amino acid response to cadmium ion

Process(es)

magnesium ion binding glutathione synthase activity protein binding ATP binding glycine binding protein homodimerization activity glutathione binding