Abnova

GTF2A2 Monoclonal Antibody (2B9)

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GTF2A2 Antibody in Western Blot (WB) — Western Blot analysis of GTF2A2 expression in transfected 293T cell line by GTF2A2 monoclonal antibody (M01), clone 2B9.

Lane 1: GTF2A2 transfected lysate(12.5 KDa).
Lane 2: Non-transfected lysate.

GTF2A2 Antibody in ELISA (ELISA) — Detection limit for recombinant GST tagged GTF2A2 is approximately 0.3 ng/mL as a capture antibody.

Product Details

H00002958-M01

Applications	Tested Dilution	Publications
Western Blot (WB)	1-5 µg/mL	-
ELISA (ELISA)	0.3 ng/mL	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Mouse / IgG2a, kappa
Class	Monoclonal
Type	Antibody
Clone	2B9
Immunogen	GTF2A2 (AAH00287, 1 a.a. approximately 109 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	See Label
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.4
Contains	no preservative
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles

Product Specific Information

Sequence of this protein is as follows: MAYQLYRNTT LGNSLQESLD ELIQSQQITP QLALQVLLQF DKAINAALAQ RVRNRVNFRG SLNTYRFCDN VWTFVLNDVE FREVTELIKV DKVKIVACDG KNTGSNTTE

Target Information

Accurate transcription initiation on TATA-containing class II genes involves the ordered assembly of RNA polymerase II (POLR2A; MIM 180660) and the general initiation factors TFIIA, TFIIB (MIM 189963), TFIID (MIM 313650), TFIIE (MIM 189962), TFIIF (MIM 189968), TFIIG/TFIIJ, and TFIIH (MIM 189972). The first step involves recognition of the TATA element by the TATA-binding subunit (TBP; MIM 600075) and may be regulated by TFIIA, a factor that interacts with both TBP and a TBP-associated factor (TAF; MIM 600475) in TFIID. TFIIA has 2 subunits (43 and 12 kD) in yeast and 3 subunits in higher eukaryotes. In HeLa extracts, it consists of a 35-kD alpha subunit and a 19-kD beta subunit encoded by the N- and C-terminal regions of GTF2A1 (MIM 600520), respectively, and a 12-kD gamma subunit encoded by GTF2A2 (DeJong et al., 1995).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: general transcription factor IIA 2; General transcription factor IIA subunit 2; general transcription factor IIA, 2, 12kDa; TFIIA gamma subunit; TFIIA p12 subunit; TFIIA-12; TFIIA-gamma; Transcription initiation factor IIA gamma chain; Transcription initiation factor IIA subunit 2

Gene Aliases: GTF2A2; HsT18745; T18745; TF2A2; TFIIA; TFIIA-12; TFIIA-gamma; TFIIAS

UniProt ID: (Human) P52657

Entrez Gene ID: (Human) 2958

Molecular Function: general transcription factor