Invitrogen

GTF2F2 Recombinant Rabbit Monoclonal Antibody (JE56-15)

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GTF2F2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry (Paraffin) analysis of paraffin-embedded human placenta tissue using GTF2F2 Recombinant Monoclonal Antibody (Product # MA5-42489). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the GTF2F2 antibody at a dilution of 1:50 for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

GTF2F2 Antibody in Western Blot (WB) — Western blot analysis of GTF2F2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. GTF2F2 Recombinant Monoclonal Antibody (Product # MA5-42489) at 1:1,000 was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: Rat testis tissue lysate. Lane 2: A549 cell lysate. Lane 3: HepG2 cell lysate. Lane 4: HL-60 cell lysate. Lane 5: THP-1 cell lysate. Lane 6: Daudi cell lysate.

Product Details

MA5-42489

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:200	-

Product Specifications
Species Reactivity	Human, Rat
Host/Isotype	Rabbit / IgG
Class	Recombinant Monoclonal
Type	Antibody
Clone	JE56-15
Immunogen	Synthetic peptide within C-terminal Human GTF2F2 View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Protein A
Storage buffer	TBS, pH 7.4, with 40% glycerol
Contains	0.05% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2911630

Product Specific Information

Positive Control: Rat testis tissue lysate, A549 cell lysate, HepG2 cell lysate, HL-60 cell lysate, THP-1 cell lysate, Daudi cell lysate, rat testis tissue, human skin tissue, human placenta tissue, human uterus tissue.

Subcellular Location: Nucleus.

Target Information

GTF2F2 is required for both basal and HIV-1 Tat-activated transcription. GTF2F2 synergizes with HIV-1 Tat and the cellular co-activator Tat-SF1 during Tat-mediated transactivation of the HIV-1 LTR promoter.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: ATP-dependent helicase GTF2F2; General transcription factor IIF 30 kDa subunit; General transcription factor IIF subunit 2; general transcription factor IIF, polypeptide 2 (30kD subunit); general transcription factor IIF, polypeptide 2, 30kDa; TFIIF beta; TFIIF-beta; transcription factor gamma; Transcription initiation factor IIF subunit beta; Transcription initiation factor RAP30

Gene Aliases: BTF4; GTF2F2; RAP30; TF2F2; TFIIF

UniProt ID: (Human) P13984

Entrez Gene ID: (Human) 2963, (Rat) 81674

Molecular Function: general transcription factor