Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is brain tissue.
Soluble guanylyl cyclase catalyses in a Mg2+ or Mn2+ dependent manner, the conversion of guanosine-5'-triphosphate (GTP) to cyclic guanosine-3',5-monophosphate (cGMP) and pyrophosphate. This enzyme is a major physiological receptor for nitric oxide (NO), an important intra- and intercellular membrane-permeant signaling molecule.
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Protein Aliases: GC-S-beta-1; GC-SB3; GCS-beta-1; GCS-beta-3; guanylate cyclase 1, soluble, beta 3; Guanylate cyclase soluble subunit beta-1; Guanylate cyclase soluble subunit beta-3; guanylate cyclase, soluble, beta 1; GUC1B3; GUCB3; GUCSB3; GUCY1B1; Soluble guanylate cyclase small subunit
Gene Aliases: GC-S-beta-1; GC-SB3; GUC1B3; GUCB3; GUCSB3; GUCY1B1; GUCY1B3; SGC