|Tested species reactivity||Human|
|Published species reactivity||Sheep|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide (KLH conjugated) to the 7th transmembrane domain.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.7|
|Contains||0.01% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:200|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 1 publications below|
OPA1-15410 detects GalR2 from human samples.
OPA1-15410 has been successfully used in immunohistochemistry procedures. By immunohistochemistry, OPA1-15410 detects GalR2 in formalin fixed, paraffin embedded human tissues.
The OPA1-15410 immunogen is a synthetic peptide (KLH conjugated) corresponding to the 7th transmembrane domain.
Store at 4°C short term. For long term storage, aliquot and store at -20°C, avoiding freeze/thaw cycles.
GalR2 expression has been documented widely in the brain and in peripheral tissues including small intestine, heart, kidney, and liver. Localization data from rat CNS indicate high levels of expression in dorsal root ganglia, amygdala, and Purkinje neurons.
G-protein Coupled Receptors (GPCRs) comprise one of the largest families of signaling molecules with more than a thousand members currently predicted to exist. All GPCRs share a structural motif consisting of seven membrane-spanning helices, and exist in both active and inactive forms. An array of activating ligands participate in the conformation of GPCRs which leads to signaling via G-proteins and downstream effectors. Ongoing studies have also shown the vast series of reactions which participate in the negative regulation of GPCRs. This "turn-off" activity has tremendous implications for the physiological action of the cell, and continues to drive pharmacological research for new drug candidates. Two blockbuster drugs which have been developed as GPCR-targeted pharmaceuticals are Zyprexa (Eli Lilly) and Claritin (Schering-Plough) which have multi-billion dollar shares of the mental health and allergy markets, respectively.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Distribution of galanin receptor-2 immunoreactive neurones in the ovine hypothalamus: no evidence for involvement in the control of gonadotrophin-releasing hormone secretion.
OPA1-15410 was used in immunohistochemistry to study the localization of galanin receptor-2 positive neurons in the ovine hypothalamus and its involvement in the secretion of gonadotrophin-releasing hormone
|Chambers G,Whitelaw CM,Robinson JE,Evans NP||Journal of neuroendocrinology (19:966)||2007|