Invitrogen

Galectin 1 Monoclonal Antibody (6C8.4-1)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

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Antibody Testing Data (8)
Advanced Verification (4)

Galectin 1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 12

Galectin 1 Antibody (43-7400) in ICC/IF

Immunofluorescence analysis of Galectin 1 was performed using 70% confluent log phase MDA-MB-231 and MCF7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Galectin 1 Monoclonal Antibody (6C8.4-1) (Product # 43-7400) at 1:100 in 0.1% BSA, incubated ... View More

Product Image Gallery

Galectin 1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of Galectin 1 was performed using 70% confluent log phase MDA-MB-231 and MCF7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with Galectin 1 Monoclonal Antibody (6C8.4-1) (Product # 43-7400) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32766), (1:2500 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic and membrane localization of Galectin 1 in MDA-MB-231. Panel e represents MCF7 cells showing negative staining for the same. Panel f represents control MDA-MB-231 cells with no primary antibody to assess background. The images were captured at 60X magnification.

Galectin 1 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of Galectin 1 was done on 70% confluent log phase U-87MG cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Galectin 1 (6C8.4-1) Mouse Monoclonal Antibody (Product # 43-7400) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d is a merged image showing cytoplasmic localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.

Galectin 1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Galectin 1 showing staining in the cytoplasm and nucleus of paraffin-embedded human esophagus tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Anti- Galectin 1 Monoclonal Antibody (Product # 43-7400) diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

Galectin 1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Galectin 1 showing staining in the cytoplasm and nucleus of paraffin-embedded human kidney tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Anti- Galectin 1 Monoclonal Antibody (Product # 43-7400) diluted in 3% BSA-PBS at a dilution of 1:100 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

Galectin 1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Galectin 1 showing staining in the cytoplasm and nucleus of paraffin-embedded mouse ovary tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Anti- Galectin 1 Monoclonal Antibody (Product # 43-7400) diluted in 3% BSA-PBS at a dilution of 1:50 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

Galectin 1 Antibody in Western Blot (WB) — Western blot was performed using Anti-Galectin 1 Monoclonal Antibody (6C8.4-1) (Product # 43-7400) and a 15kDa band corresponding to Galectin 1 was observed across PC-3, THP-1, MDA-MB-231 but not in LNCaP and MCF7 and in Mouse Adipose. Whole cell extracts (30 µg lysate) of PC-3 (Lane 1), LNCaP (Lane 2), THP-1 (Lane 3), MDA-MB-231 (Lane 4), MCF7 (Lane 5) and tissue extracts (30 ug lysate) of Mouse Adipose (Lane 6) were electrophoresed using NuPAGE™ 12% Bis-Tris Protein Gel (Product # NP0341BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177,1:8000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005). Increased expression of Galectin-1 is seen in triple negative breast cancer (TNCB) (like MDA-MB-231) for promoting metastasis but not in non-TNBC like MCF7 [10.18632/oncotarget.16208] and in androgen unresponsive PC-3 cells in comparison to androgen responsive LNCaP [10.1155/2013/519436]. The band at ~25 kDa in Mouse Adipose corresponds to circulating IgGs that are commonly detected in mouse tissue lysates.

Galectin 1 Antibody in Western Blot (WB) — Western blot analysis was performed on whole cell extracts (30 µg lysate) of THP-1. The blots were probed with Anti-Galectin 1 Mouse Monoclonal Antibody (Product # 43-7400, 1-2 µg/mL) and detected by chemiluminescence Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP conjugate (Product # 62-6520, 1:4000 dilution). A 14 kDa band corresponding to Galectin 1 was observed across cell lines tested.Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane using iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

Galectin 1 Antibody in Western Blot (WB) — Knockdown of Galectin 1 was achieved by transfecting PC-3 with Galectin 1 specific siRNAs (Silencer® select Product # s194592, s8145). Western blot analysis (Fig. a) was performed using whole cell extracts (40 ug lysate) from the Galectin 1 knockdown cells (Lane 3), non-targeting scrambled siRNA transfected cells (Lane 2) and untransfected cells (Lane 1). The blot was probed with Galectin 1 Monoclonal Antibody (6C8.4-1) (Product # 43-7400, 1:1000 dilution ) and Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:8000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to Galectin 1.

Galectin 1 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. PC-3 cells were transfected with Galectin 1 siRNA and decrease in signal intensity was observed in Western Blot application using Anti-Galectin 1 Monoclonal Antibody (6C8.4-1) (Product # 43-7400). {KD}

Galectin 1 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines owing to their inherent genetic constitution. Relative expression of Galectin 1 was observed in MDA-MB-231 in comparison to MCF7 and PC-3 to LNCaP owing to their metastatic potential and androgen response capacity, respectively, using Anti-Galectin 1 Monoclonal Antibody (6C8.4-1) (Product # 43-7400) in Western Blot. {RE}

Galectin 1 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell models owing to their inherent genetic constitution. Immunofluorescence analysis using Anti-Galectin 1 Monoclonal Antibody (6C8.4-1) (Product # 43-7400), shows cytoplasmic and membrane staining for Galectin 1 in MDA-MB-231 cells, a triple negative breast cancer cell line, in comparison to non-TNBC cell line MCF7. {RE}

Galectin 1 Antibody — Figure 6 Hypoxia causes exacerbation of fibrosis via galectin-1 in mice injured with low levels of bleomycin. ( A ) Experimental design depicting two bleomycin insults (day 0--D0; day 7--D7) followed by hypoxia treatment for 3 weeks (day 8 to day 28). Galectin-1 inhibitor (OTX008) treatment was performed from day 5 to day 28. ( B ) Hypoxia treatment of mice injured with bleomycin induced fibrosis. Mice were exposed to hypoxia alone (m-p) or treated with intratracheal bleomycin alone (e-h) or underwent hypoxia and bleomycin insults together (q-t). Mice were also treated with galectin-1 inhibitor (i-l, u-x) or saline (e-h, q-t). H&E staining (a, e, i, m, q, u), Masson's trichrome staining for visualization of collagen deposition (b, f, j, n, r, v), alpha -SMA staining (c, g, k, o, s, w), and galectin-1 staining (d, h, l, p, t, x) was performed. Black scale bars=100 mu m; blue scale bars=20 mu m. alpha -SMA expression is increased in the proximal parenchyma (s; arrows) and distal parenchyma (s, inset) of mouse lungs treated with hypoxia and bleomycin. Similarly, galectin-1 expression is increased in proximal parenchyma (t; arrow and right bottom inset) and distal parenchyma (left bottom inset). Galectin-1 inhibition reversed lung remodeling (u), collagen deposition (v), and reduced alpha -SMA (w) and galectin-1 expression (x). ( C ) Galectin-1 inhibition reduced total collagen deposition in the lungs of mice injured with hypoxia and bleomycin. Total collagen was measured using s {TM}

Product Details

43-7400

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 4 publications 4 publications

Immunohistochemistry (IHC)

-

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20

-

Immunocytochemistry (ICC/IF)

1:100

View 1 publication 1 publication

Flow Cytometry (Flow)

-

View 1 publication 1 publication

ELISA (ELISA)

-

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse, Pig

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

6C8.4-1

Immunogen

Mixture of two peptides from the middle and C-terminal regions of human Galectin-1 protein

Conjugate

Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2533538

Product Specific Information

The peptides used for immunization have ~90% similarity to swine, rat, ovine, Chinese hamster sequences and 80% similar to bovine and mouse sequences.

Target Information

Galectin-1 belongs to a large family of carbohydrate-binding proteins called lectins. Galectin-1can be either monomeric or homodimeric and is found in a wide variety of cells and tissue types. Galectin-1 can control cell growth, proliferation, induce apoptosis of activated T cells while it can also modulate cytokine secretion or inhibit pro-inflammatory cytokine production. Galectin-1 plays an important role in acute and chronic inflammation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Stromal Galectin-1 Promotes Colorectal Cancer Cancer-Initiating Cell Features and Disease Dissemination Through SOX9 and β-Catenin: Development of Niche-Based Biomarkers.

Frontiers in oncology

Peng KY,Jiang SS,Lee YW,Tsai FY,Chang CC,Chen LT,Yen BL

43-7400 was used in Western Blot, Immunohistochemistry, ELISA to elucidate the molecular interactions between stromal and CRC cells for the development of prognostically relevant biomarkers.

Wed Sep 29 00:00:00 EDT 2021

Stromal Galectin-1 Promotes Colorectal Cancer Cancer-Initiating Cell Features and Disease Dissemination Through SOX9 and β-Catenin: Development of Niche-Based Biomarkers.

Frontiers in oncology

Peng KY,Jiang SS,Lee YW,Tsai FY,Chang CC,Chen LT,Yen BL

43-7400 was used in Western Blot, Immunohistochemistry, ELISA to elucidate the molecular interactions between stromal and CRC cells for the development of prognostically relevant biomarkers.

Wed Sep 29 00:00:00 EDT 2021

Stromal Galectin-1 Promotes Colorectal Cancer Cancer-Initiating Cell Features and Disease Dissemination Through SOX9 and β-Catenin: Development of Niche-Based Biomarkers.

Frontiers in oncology

Peng KY,Jiang SS,Lee YW,Tsai FY,Chang CC,Chen LT,Yen BL

43-7400 was used in Western Blot, Immunohistochemistry, ELISA to elucidate the molecular interactions between stromal and CRC cells for the development of prognostically relevant biomarkers.

Wed Sep 29 00:00:00 EDT 2021

Stromal Galectin-1 Promotes Colorectal Cancer Cancer-Initiating Cell Features and Disease Dissemination Through SOX9 and β-Catenin: Development of Niche-Based Biomarkers.

Frontiers in oncology

Peng KY,Jiang SS,Lee YW,Tsai FY,Chang CC,Chen LT,Yen BL

43-7400 was used in Western Blot, Immunohistochemistry, ELISA to elucidate the molecular interactions between stromal and CRC cells for the development of prognostically relevant biomarkers.

Wed Sep 29 00:00:00 EDT 2021

Stromal Galectin-1 Promotes Colorectal Cancer Cancer-Initiating Cell Features and Disease Dissemination Through SOX9 and β-Catenin: Development of Niche-Based Biomarkers.

Frontiers in oncology

Peng KY,Jiang SS,Lee YW,Tsai FY,Chang CC,Chen LT,Yen BL

43-7400 was used in Western Blot, Immunohistochemistry, ELISA to elucidate the molecular interactions between stromal and CRC cells for the development of prognostically relevant biomarkers.

Wed Sep 29 00:00:00 EDT 2021

Stromal Galectin-1 Promotes Colorectal Cancer Cancer-Initiating Cell Features and Disease Dissemination Through SOX9 and β-Catenin: Development of Niche-Based Biomarkers.

Frontiers in oncology

Peng KY,Jiang SS,Lee YW,Tsai FY,Chang CC,Chen LT,Yen BL

43-7400 was used in Western Blot, Immunohistochemistry, ELISA to elucidate the molecular interactions between stromal and CRC cells for the development of prognostically relevant biomarkers.

Wed Sep 29 00:00:00 EDT 2021

Therapeutic Benefit of Galectin-1: Beyond Membrane Repair, a Multifaceted Approach to LGMD2B.

Cells

Vallecillo-Zúniga ML,Poulson PD,Luddington JS,Arnold CJ,Rathgeber M,Kartchner BC,Hayes S,Gill H,Valdoz JC,Spallino JL,Garfield S,Dodson EL,Arthur CM,Stowell SR,Van Ry PM

Published figure using Galectin 1 monoclonal antibody (Product # 43-7400) in Western Blot

Wed Nov 17 00:00:00 EST 2021

Therapeutic Benefit of Galectin-1: Beyond Membrane Repair, a Multifaceted Approach to LGMD2B.

Cells

Vallecillo-Zúniga ML,Poulson PD,Luddington JS,Arnold CJ,Rathgeber M,Kartchner BC,Hayes S,Gill H,Valdoz JC,Spallino JL,Garfield S,Dodson EL,Arthur CM,Stowell SR,Van Ry PM

Published figure using Galectin 1 monoclonal antibody (Product # 43-7400) in Western Blot

Wed Nov 17 00:00:00 EST 2021

New Treatment Strategy Targeting Galectin-1 against Thyroid Cancer.

Cells

Gheysen L,Soumoy L,Trelcat A,Verset L,Journe F,Saussez S

43-7400 was used in Immunocytochemistry to evaluate the impacts of OTX008 in six thyroid cancer cell lines.

Wed May 05 00:00:00 EDT 2021

Treatment with galectin-1 improves myogenic potential and membrane repair in dysferlin-deficient models.

PloS one

Vallecillo-Zúniga ML,Rathgeber MF,Poulson PD,Hayes S,Luddington JS,Gill HN,Teynor M,Kartchner BC,Valdoz J,Stowell C,Markham AR,Arthur C,Stowell S,Van Ry PM

Published figure using Galectin 1 monoclonal antibody (Product # 43-7400) in Western Blot

Tue Oct 27 00:00:00 EDT 2020

Treatment with galectin-1 improves myogenic potential and membrane repair in dysferlin-deficient models.

PloS one

Vallecillo-Zúniga ML,Rathgeber MF,Poulson PD,Hayes S,Luddington JS,Gill HN,Teynor M,Kartchner BC,Valdoz J,Stowell C,Markham AR,Arthur C,Stowell S,Van Ry PM

Published figure using Galectin 1 monoclonal antibody (Product # 43-7400) in Western Blot

Tue Oct 27 00:00:00 EDT 2020

Sum of peak intensities outperforms peak area integration in iTRAQ protein expression measurement by LC-MS/MS using a TripleTOF 5600+ platform.

Bioscience reports

Burat B,Gonzalez J,Sauvage FL,Aouad H,Arnion H,Pinault E,Marquet P,Essig M

43-7400 was used in Western Blotting to propose the Mascot-jTRAQx-CiR-C strategy as a simple yet powerful data processing adjunct to the iTRAQ technology.

Fri Jun 28 00:00:00 EDT 2019

Galectin-1 inhibition attenuates profibrotic signaling in hypoxia-induced pulmonary fibrosis.

Cell death discovery

Kathiriya JJ,Nakra N,Nixon J,Patel PS,Vaghasiya V,Alhassani A,Tian Z,Allen-Gipson D,Davé V

Published figure using Galectin 1 monoclonal antibody (Product # 43-7400) in Immunohistochemistry

Thu Mar 31 00:00:00 EDT 2022

Galectin-1 inhibition attenuates profibrotic signaling in hypoxia-induced pulmonary fibrosis.

Cell death discovery

Kathiriya JJ,Nakra N,Nixon J,Patel PS,Vaghasiya V,Alhassani A,Tian Z,Allen-Gipson D,Davé V

Published figure using Galectin 1 monoclonal antibody (Product # 43-7400) in Immunohistochemistry

Thu Mar 31 00:00:00 EDT 2022

Contact-dependent interference with invariant NKT cell activation by herpes simplex virus-infected cells.

Journal of immunology (Baltimore, Md. : 1950)

Bosnjak L,Sahlström P,Paquin-Proulx D,Leeansyah E,Moll M,Sandberg JK

43-7400 was used in flow cytometry to examine the role of human iNKT cells in HSV-1 infection and study their interaction with epidermal keratinocytes

Fri Jun 15 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: 14 kDa laminin-binding protein; 14 kDa lectin; beta-galactoside binding protein; Beta-galactoside-binding lectin L-14-I; beta-galactoside-binding protein 14kDa; DKFZp686E23103; Gal-1; Galaptin; Galectin-1; Galectin1; H-Galectin-1; HBL; HLBP14; HPL; Lactose-binding lectin 1; Lectin galactoside-binding soluble 1; lectin, galactoside binding soluble 1; lectin, galactoside-binding, soluble, 1; pm12; Putative MAPK-activating protein PM12; S-Lac lectin 1

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Gene Aliases: Gbp; LGALS1

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Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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