|Tested species reactivity||Human, Mouse|
|Published species reactivity||Not Applicable|
|Host / Isotype||Mouse / IgG1|
|Immunogen||First 45 residues of human Galectin-3.|
|Storage buffer||PBS with 0.1% BSA|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:50|
|Functional Assay (FN)||1:50|
|Immunohistochemistry (Frozen) (IHC (F))||1:50|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50|
|Western Blot (WB)||1:50|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 1 publications below|
MA1-40229 detects Galectin-3 from human and mouse samples. This antibody does not cross react with Galedin-3C and is cross reactive with mouse galectin-3.
MA1-40229 has been successfully used in flow cytometry, immunohistochemistry (frozen), immunohistochemistry (paraffin), functional assays, ELISA and Western blot applications.
The MA1-40229 immunogen is the first 45 residues of human Galectin-3.
The monolonal antibody B2C10 reacts with galectin-3, a 30 kDa protein. Galectin-3 is a member of the galectin family. The protein is compose of three domains: a small amino-terminal domain, a carboxyl-terminal carbohydrate recognition domain (CRD) and amino-terminal domain containing repeating elements. Galectin-3 is normally distributed in epithelia of many organs and various inflammatory cells, including macrophages, as well as dendritic cells and Kupffer cells. The expression of this lectin is up-regulated during inflammation, cell proliferation, cell differentiation and through trans-activation by viral proteins. The expression is also affected by neoplastic transformation: up-regulated in certain types of lymphomas and thyroid carcinoma, while down-regulated in other types of malignancies, such as colon, breast, ovarian and uterine carcinomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Not Applicable||Not Cited||
Modulation of functional properties of galectin-3 by monoclonal antibodies binding to the non-lectin domains.
MA1-40229 was used in blocking/activating experiment, ELISA and western blot to produce and characterize novel anti-galectin 3 monoclonal antibodies
|Liu FT,Hsu DK,Zuberi RI,Hill PN,Shenhav A,Kuwabara I,Chen SS||Biochemistry (35:6073)||1996|