Immunofluorescent analysis of Gal4 (green) in Gal4 DNA Binding Domain expressing 293T cells (Product # K1091A). The cells were fixed with formalin for 20 minutes, permeabilized with 0.1% Triton X-100 in TBS for 15 minutes, and blocked with 5% Normal Goat Serum (Product # 31872) for 15 minutes at room temperature. Cells were stained with or without Gal4 mouse monoclonal antibody (Product # 33-8600), at a dilution of 1:50 overnight at 4C, and then incubated with a DyLight 488 goat anti-mouse IgG secondary antibody (Product # 35503) at a dilution of 1:1000 for 30 minutes at room temperature (both panels, green). Nuclei (both panels, blue) were stained with Hoechst 33342 dye (Product # 62249). Images were taken on a Thermo Scientific ToxInsight at 20X magnification.
|Tested species reactivity||Yeast|
|Published species reactivity||Mouse|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||A recombinant protein corresponding to amino acids 1-147 mapping within the amino terminal, DNA binding domain of GAL4.|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunocytochemistry (ICC)||1:50 - 1:100|
|Immunofluorescence (IF)||1:50 - 1:100|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunofluorescence (IF)||See 1 publications below|
33-8600 detects the Gal4 DNA Binding Domain by western blot, immunoprecipitation and immunofluorescence corresponding to amino acids 1-147 of the GAL4 DNA binding region.
The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. The expression of this gene is restricted to small intestine, colon, and rectum, and it is underexpressed in colorectal cancer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
|Mouse||2 µg/ml||Intramembranous valine linked to schizophrenia is required for neuregulin 1 regulation of the morphological development of cortical neurons.||Chen Y,Hancock ML,Role LW,Talmage DA||The Journal of neuroscience : the official journal of the Society for Neuroscience (30:9199)||2010|