Note: You clicked on an external link, which has been disabled in order to keep your shopping session open.
Immunofluorescence analysis of Gamma-Catenin was performed using 70% confluent log phase CACO2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Gamma-Catenin / Plakoglobin (PG-11E4) Mouse Monoclonal Antibody (Product # 13-8500) at 2µg/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjµgate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Human, Mouse|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Fusion protein consisting of the maltose binding protein fused to full length human plakoglobin protein.|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Immunohistochemistry (IHC)||Assay Dependent|
|Immunoprecipitation (IP)||Assay Dependent|
|Western Blot (WB)||2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
JUP is a major cytoplasmic protein which is the only known constituent common to submembranous plaques of both desmosomes and intermediate junctions. This protein forms distinct complexes with cadherins and desmosomal cadherins and is a member of the catenin family since it contains a distinct repeating amino acid motif called the armadillo repeat.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Glucocorticoids and histone deacetylase inhibitors cooperate to block the invasiveness of basal-like breast cancer cells through novel mechanisms.
13-8500 was used in immunocytochemistry and western blot to report that glucocorticoids and HDAC inhibitors cooperate to suppress the invasiveness of breast cancer cells via E-cadherin.
|Law ME,Corsino PE,Jahn SC,Davis BJ,Chen S,Patel B,Pham K,Lu J,Sheppard B,Nørgaard P,Hong J,Higgins P,Kim JS,Luesch H,Law BK||Oncogene (32:1316)||2013|
EpCAM contributes to formation of functional tight junction in the intestinal epithelium by recruiting claudin proteins.
13-8500 was used in immunohistochemistry (frozen) to report that mice lacking EpCAM have intestinal barrier defects and die shortly after birth as a result of intestinal erosion.
|Lei Z,Maeda T,Tamura A,Nakamura T,Yamazaki Y,Shiratori H,Yashiro K,Tsukita S,Hamada H||Developmental biology (371:136)||2012|
SOX4 interacts with plakoglobin in a Wnt3a-dependent manner in prostate cancer cells.
13-8500 was used in immunoprecipitation to identify SOX4 binding partners.
|Lai YH,Cheng J,Cheng D,Feasel ME,Beste KD,Peng J,Nusrat A,Moreno CS||BMC cell biology (12:null)||2011|
|Human||1:100||Mispolarization of desmosomal proteins and altered intercellular adhesion in autosomal dominant polycystic kidney disease.||Silberberg M,Charron AJ,Bacallao R,Wandinger-Ness A||American journal of physiology. Renal physiology (288:F1153)||2005|
Tumor-derived mutated E-cadherin influences beta-catenin localization and increases susceptibility to actin cytoskeletal changes induced by pervanadate.
13-8500 was used in western blot to assess the localization of adherens junction components in cells expressing mutant E-cadherin derivatives.
|Luber B,Candidus S,Handschuh G,Mentele E,Hutzler P,Feller S,Voss J,Höfler H,Becker KF||Cell adhesion and communication (7:391)||2000|
catenin (cadherin-associated protein), gamma 80kDa; CTNNG; desmoplakin III; desmoplakin-3; DP3; gamma-catenin; junction plakoglobin; JUP
ARVD12; CTNNG; D930025P04Rik; DP3; DPIII; JUP; PDGB; PG; PKGB