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Invitrogen
Ganglioside GM2 Chimeric Recombinant Rabbit Monoclonal Antibody (L55-81)
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Ganglioside GM2 Chimeric Antibody (MA5-48069) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis of Ganglioside GM2 in MCF7 cells using Ganglioside GM2 Chimeric Monoclonal Antibody (Product # MA5-48069). Paraformaldehyde-fixed cells were immobilized on cover slips and stained with Ganglioside GM2 Chimeric Monoclonal Antibody at 10 µg/mL for 1h followed by Alexa Fluor488 secondary antibody (1 µg/mL), showing diffuse membrane and cytoplasmic staining. The n... View More
Product Details
MA5-48069
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG, kappa
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
L55-81
Immunogen
L55-81 was prepared by isolating EBV-immortalized peripheral blood B lymphocytes from a human donor with melanoma.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS
Contains
0.02% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_3074512
Product Specific Information
Specificity: L55-81 binds specifically to GM2 ganglioside; this antibody shows no reactivity towards GM1, GM3, GD2 or normal tissues- the L55-81 epitope contains both a GalNAc and sialic acid bound to lactose. Ganglioside GM2 (GalNAcBeta1-4(NeuNAcalpha2-3)GalBeta1-4GlcBeta-1ceramide) is a glycosphingolipid present on the cell surface and a major tumor-differentiation antigen in human melanoma and hepatoma.
Target Information
Ganglioside GM2 is a glycosphingolipid found in the plasma membrane of cells, particularly in the nervous system. It is composed of a ceramide lipid tail and a complex carbohydrate head group that contains sialic acid residues. The function of Ganglioside GM2 is not fully understood, but it is believed to play a role in cell signaling and communication, as well as in the formation and maintenance of the myelin sheath that surrounds nerve cells. Mutations in the genes responsible for the synthesis or degradation of Ganglioside GM2 can result in various neurological disorders, including Tay-Sachs disease and Sandhoff disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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