|Tested species reactivity||Human|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide (KLH conjugated) to the N-terminus.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.7|
|Contains||0.01% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:200|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 1 publications below|
OPA1-15060 detects the gastric inhibitory polypeptide receptor from human samples.
OPA1-15060 has been successfully used in immunohistochemistry procedures. By immunohistochemistry, OPA1-15060 detects the gastric inhibitory polypeptide receptor in formalin fixed, paraffin embedded human tissues.
The OPA1-15060 immunogen is a synthetic peptide (KLH conjugated) to the N-terminus.
GIPR, or Gastric Inhibitory Polypeptide Receptor, mediates GIP-induced secretion of insulin by pancreatic islet beta cells after a meal. GIP is a gastrointestinal peptide hormone of 42 aa that is released from duodenal endocrine K cells after absorption of glucose or fat. Stimulation of the GIPR on pancreatic cells activates adenylyl cyclase and mitogen-activated protein kinase, resulting in increased insulin secretion. Mice with a targeted mutation of GIPR have higher blood glucose levels with impaired initial insulin response after oral glucose load. Analysis of GIPR knockout mice suggest that GIPR defects may contribute to the pathogenesis of diabetes and obesity. Ectopic expression of functional GIPR and its coupling to steroidogenesis has been suggested to be the main cause of food-dependent Cushinga€(tm)s syndrome. Two isoforms of GIPR are produced by alternative splicing. GIPR expression has been reported in human bone, fetal adrenal, and pancreas. Little expression has been identified in normal adult adrenal, but overexpression of GIPR has been observed in the adrenal in food-dependent Cushing's syndrome. GIPR expression has been identified in rat brain, heart, pancreas, and small intestine. ESTs have been isolated from colon libraries.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Expression profiles of the glucose-dependent insulinotropic peptide receptor and LHCGR in sporadic adrenocortical tumors.
OPA1-15060 was used in immunohistochemistry to study the expression levels of GIPR and LHCGR in sporadic adrenocortical tumors form Brazilian population
|Costa MH,Latronico AC,Martin RM,Barbosa AS,Almeida MQ,Lotfi CF,Valassi HP,Nishi MY,Lucon AM,Siqueira SA,Zerbini MC,Carvalho LR,Mendonca BB,Fragoso MC||The Journal of endocrinology (200:167)||2009|