Invitrogen

Gata-3 Monoclonal Antibody (TWAJ), Alexa Fluor™ 488, eBioscience™

Gata-3 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 29

Gata-3 Antibody (53-9966-42) in ICC/IF

Knockdown of GATA-3 was achieved by transfecting MCF7 cells with GATA-3 specific siRNAs (Silencer® select Product # s5600; s5599). Immunofluorescence analysis was performed using untransfected MCF7 (panels a, d), transfected with non-specific scrambled siRNA (panels b,e) and transfected with GATA-3 specific siRNAs (panel c,f). The cells were labeled with GATA-3 Monoclonal Antibody (TWAJ), Alexa Fluor 488, (Product # 53-9966-41) at 1:250 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight (Panel a: green). Nuclei (blue) were stain... View More

Gata-3 Antibody in Flow Cytometry (Flow)

Gata-3 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

Product Details

53-9966-42

Applications

Tested Dilution

Publications

Western Blot (WB)

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

1:250

Flow Cytometry (Flow)

5 µL (0.125 µg)/test

View 23 publications 23 publications

Product Specifications

Species Reactivity

Human, Mouse, Pig, Rhesus monkey

Published species

Human, Mouse

Host/Isotype

Rat / IgG2b, kappa

Recommended Isotype Control

Rat IgG2b kappa Isotype Control (eB149/10H5), Alexa Fluor™ 488, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

TWAJ

Conjugate

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

Excitation/Emission Max

499/520 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with BSA

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2574493

Product Specific Information

Description: The monoclonal antibody TWAJ recognizes mouse and human Gata-3, a member of the Gata family of transcription factors. Gata-3 is a T cell-specific transcription factor important for thymic development and Th2 differentiation. Expression during embryonic development is found in the central nervous system, skin, mammary glands and kidney. During development, the expression of Gata-3 is essential as homozygous knock-out of Gata-3 is embryonic lethal. The Gata-3 is also essential for T cell commitment and survival. In the thymus, expression is found mainly on the CD4 single positive cells. During Th2 differentiation, Gata-3 binds to the IL-4 promoter as well as represses the expression of T-bet, thus inhibiting Th1 differentiation.

Alternative splice variants have been reported especially in the MCF7 cell line. The TWAJ Human/Mouse Gata-3 antibody will recognize both forms (50 and 45 kDa) of the protein.

Staining with the TWAJ Human/Mouse Gata-3 antibody requires the use of the Foxp3/Transcription Factor Staining Buffer Set.(Product # 00-5523-00)

Cross reactivity in rhesus monkeys has been published.

Applications Reported: This TWAJ antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This TWAJ antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of mouse thymocytes using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523) and protocol. Please refer to BestProtocols®: Protocol B: One step protocol for (nuclear) intracellular proteins located under the Resources Tab online. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The genes for all 4 subunits of the T-cell antigen receptor (alpha, beta, gamma and delta) are controlled by distinct enhancers and their enhancer-binding proteins. Marine and Winoto (1991) identified a common TCR regulatory element by demonstrating binding of the enhancer-binding protein GATA3 to the enhancer elements of all 4 TCR genes. GATA3 had been shown in the chicken to be an enhancer-binding protein containing a zinc finger domain. GATA3 mRNA was demonstrated by Northern blot analysis in T cells but not in B cells or macrophages. GATA3 is abundantly expressed in the T-lymphocyte lineage and is thought to participate in T-cell receptor gene activation through binding to enhancers. Labastie et al. (1994) cloned the human gene and the 5-prime end of the mouse gene. The human gene comprises 6 exons distributed over 17 kb of DNA. Its 2 zinc fingers are encoded by 2 separate exons highly conserved with those of GATA1, but no other structural homologies between the 2 genes could be found.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Th2 cells inhibit growth of colon and pancreas cancers by promoting anti-tumorigenic responses from macrophages and eosinophils.

British journal of cancer

Jacenik D,Karagiannidis I,Beswick EJ

Published figure using Gata-3 monoclonal antibody (Product # 53-9966-42) in Immunohistochemistry

Sun Jan 01 00:00:00 UTC 2023

Invasive margin tissue-resident macrophages of high CD163 expression impede responses to T cell-based immunotherapy.

Journal for immunotherapy of cancer

van Elsas MJ,Labrie C,Etzerodt A,Charoentong P,van Stigt Thans JJC,Van Hall T,van der Burg SH

53-9966-42 was used in Flow Cytometry to identify a small but discernible CD163hi macrophage population, with high expression of several tumor-promoting macrophage markers and a functional anti-inflammatory transcriptome profile, but not other macrophages, to be responsible.

Wed Mar 01 00:00:00 UTC 2023

GM-CSF-activated human dendritic cells promote type 1 T follicular helper cell polarization in a CD40-dependent manner.

Journal of cell science

Korniotis S,Saichi M,Trichot C,Hoffmann C,Amblard E,Viguier A,Grondin S,Noel F,Mattoo H,Soumelis V

Published figure using Gata-3 monoclonal antibody (Product # 53-9966-42) in Flow Cytometry

Tue Nov 01 00:00:00 UTC 2022

Airway Administration of Bacterial Lysate OM-85 Protects Mice Against Respiratory Syncytial Virus Infection.

Frontiers in immunology

Antunes KH,Cassão G,Santos LD,Borges SG,Poppe J,Gonçalves JB,Nunes EDS,Recacho GF,Sousa VB,Da Silva GS,Mansur D,Stein RT,Pasquali C,De Souza APD

Published figure using Gata-3 monoclonal antibody (Product # 53-9966-42) in Flow Cytometry

Tue May 24 00:00:00 UTC 2022

The molecular and epigenetic mechanisms of innate lymphoid cell (ILC) memory and its relevance for asthma.

The Journal of experimental medicine

Verma M,Michalec L,Sripada A,McKay J,Sirohi K,Verma D,Sheth D,Martin R,Dyjack N,Seibold MA,Knapp JR,Tu TH,O'Connor BP,Gorska MM,Alam R

Published figure using Gata-3 monoclonal antibody (Product # 53-9966-42) in Flow Cytometry

Mon Jul 05 00:00:00 UTC 2021

Dysregulated Peripheral Invariant Natural Killer T Cells in Plaque Psoriasis Patients.

Frontiers in cell and developmental biology

Hu Y,Chen Y,Chen Z,Zhang X,Guo C,Yu Z,Xu P,Sun L,Zhou X,Gong Y,Yu Q,Shi Y

53-9966-42 was used in Flow Cytometry, Immunocytochemistry to explore the expression of iNKT cells in psoriasis patients and the effect of secukinumab on them.

Wed Feb 23 00:00:00 UTC 2022

Histone deacetylase 3 represses cholesterol efflux during CD4⁺ T-cell activation.

eLife

Wilfahrt D,Philips RL,Lama J,Kizerwetter M,Shapiro MJ,McCue SA,Kennedy MM,Rajcula MJ,Zeng H,Shapiro VS

Published figure using Gata-3 monoclonal antibody (Product # 53-9966-42) in Flow Cytometry

Thu Dec 02 00:00:00 UTC 2021

Effector T cell responses unleashed by regulatory T cell ablation exacerbate oral squamous cell carcinoma.

Cell reports. Medicine

Chao JL,Korzinkin M,Zhavoronkov A,Ozerov IV,Walker MT,Higgins K,Lingen MW,Izumchenko E,Savage PA

Published figure using Gata-3 monoclonal antibody (Product # 53-9966-42) in Flow Cytometry

Tue Sep 21 00:00:00 UTC 2021

LmjF.36.3850, a novel hypothetical Leishmania major protein, contributes to the infection.

Immunology

Zutshi S,Sarode AY,Ghosh SK,Jha MK,Sudan R,Kumar S,Sadhale LP,Roy S,Saha B

Published figure using Gata-3 monoclonal antibody (Product # 53-9966-42) in Flow Cytometry

Sun Aug 01 00:00:00 UTC 2021

A coordinated progression of progenitor cell states initiates urinary tract development.

Nature communications

Sanchez-Ferras O,Pacis A,Sotiropoulou M,Zhang Y,Wang YC,Bourgey M,Bourque G,Ragoussis J,Bouchard M

Published figure using Gata-3 monoclonal antibody (Product # 53-9966-42) in Immunohistochemistry

Tue May 11 00:00:00 UTC 2021

Graded RhoA GTPase Expression in Treg Cells Distinguishes Tumor Immunity From Autoimmunity.

Frontiers in immunology

Kalim KW,Yang JQ,Modur V,Nguyen P,Li Y,Zheng Y,Guo F

Published figure using Gata-3 monoclonal antibody (Product # 53-9966-42) in Flow Cytometry

Tue Jan 11 00:00:00 UTC 2022

Attenuation of TCR-induced transcription by Bach2 controls regulatory T cell differentiation and homeostasis.

Nature communications

Sidwell T,Liao Y,Garnham AL,Vasanthakumar A,Gloury R,Blume J,Teh PP,Chisanga D,Thelemann C,de Labastida Rivera F,Engwerda CR,Corcoran L,Kometani K,Kurosaki T,Smyth GK,Shi W,Kallies A

Published figure using Gata-3 monoclonal antibody (Product # 53-9966-42) in Flow Cytometry

Tue Jan 14 00:00:00 UTC 2020

Bendamustine with total body irradiation conditioning yields tolerant T-cells while preserving T-cell-dependent graft-versus-leukemia.

Oncoimmunology

Stokes J,Hoffman EA,Molina MS,Kummet N,Simpson RJ,Zeng Y,Katsanis E

Published figure using Gata-3 monoclonal antibody (Product # 53-9966-42) in Flow Cytometry

Wed Jul 14 00:00:00 UTC 2021

The obesity-induced adipokine sST2 exacerbates adipose T_reg and ILC2 depletion and promotes insulin resistance.

Science advances

Zhao XY,Zhou L,Chen Z,Ji Y,Peng X,Qi L,Li S,Lin JD

Published figure using Gata-3 monoclonal antibody (Product # 53-9966-42) in Flow Cytometry

Fri May 01 00:00:00 UTC 2020

Compartmentalized gut lymph node drainage dictates adaptive immune responses.

Nature

Esterházy D,Canesso MCC,Mesin L,Muller PA,de Castro TBR,Lockhart A,ElJalby M,Faria AMC,Mucida D

Published figure using Gata-3 monoclonal antibody (Product # 53-9966-42) in Flow Cytometry

Wed May 01 00:00:00 UTC 2019

The Chemoattractant Receptor Ebi2 Drives Intranodal Naive CD4⁺ T Cell Peripheralization to Promote Effective Adaptive Immunity.

Immunity

Baptista AP,Gola A,Huang Y,Milanez-Almeida P,Torabi-Parizi P,Urban JF,Shapiro VS,Gerner MY,Germain RN

53-9966 was used in Flow cytometry/Cell sorting to demonstrate evolutionary selection for distinct niches within the LN that promote cellular responses, emphasizing the critical link between fine-grained tissue organization and host defense.

Tue May 21 00:00:00 UTC 2019

Characterization of Transcriptional Regulatory Networks that Promote and Restrict Identities and Functions of Intestinal Innate Lymphoid Cells.

Immunity

Pokrovskii M,Hall JA,Ochayon DE,Yi R,Chaimowitz NS,Seelamneni H,Carriero N,Watters A,Waggoner SN,Littman DR,Bonneau R,Miraldi ER

53-9966 was used in Flow cytometry/Cell sorting to study regulatory interactions between transcription factors and genes within intestinal type 1, 2, and 3 innate lymphoid cells subsets.

Tue Jul 16 00:00:00 UTC 2019

IL-33-mediated mast cell activation promotes gastric cancer through macrophage mobilization.

Nature communications

Eissmann MF,Dijkstra C,Jarnicki A,Phesse T,Brunnberg J,Poh AR,Etemadi N,Tsantikos E,Thiem S,Huntington ND,Hibbs ML,Boussioutas A,Grimbaldeston MA,Buchert M,O'Donoghue RJJ,Masson F,Ernst M

Published figure using Gata-3 monoclonal antibody (Product # 53-9966-42) in Flow Cytometry

Fri Jun 21 00:00:00 UTC 2019

TNFR2 Signaling Enhances ILC2 Survival, Function, and Induction of Airway Hyperreactivity.

Cell reports

Hurrell BP,Galle-Treger L,Jahani PS,Howard E,Helou DG,Banie H,Soroosh P,Akbari O

Published figure using Gata-3 monoclonal antibody (Product # 53-9966-42) in Flow Cytometry

Tue Dec 24 00:00:00 UTC 2019

B-Lymphocyte Phenotype Determines T-Lymphocyte Subset Differentiation in Autoimmune Diabetes.

Frontiers in immunology

Egia-Mendikute L,Arpa B,Rosell-Mases E,Corral-Pujol M,Carrascal J,Carrillo J,Mora C,Chapman H,Panosa A,Vives-Pi M,Stratmann T,Serreze D,Verdaguer J

53-9966-42 was used in Flow cytometry/Cell sorting to evaluates the T- and B-lymphocyte phenotype in two transgenic NOD mouse models were generated: the NOD-PerIg and the 116C-NOD mice.

Tue Oct 13 00:00:00 UTC 2020

Stability and function of regulatory T cells expressing the transcription factor T-bet.

Nature

Levine AG,Mendoza A,Hemmers S,Moltedo B,Niec RE,Schizas M,Hoyos BE,Putintseva EV,Chaudhry A,Dikiy S,Fujisawa S,Chudakov DM,Treuting PM,Rudensky AY

Published figure using Gata-3 monoclonal antibody (Product # 53-9966-42) in Flow Cytometry

Thu Jun 15 00:00:00 UTC 2017

Characterisation of innate lymphoid cell populations at different sites in mice with defective T cell immunity.

Wellcome open research

Dutton EE,Camelo A,Sleeman M,Herbst R,Carlesso G,Belz GT,Withers DR

Published figure using Gata-3 monoclonal antibody (Product # 53-9966-42) in Flow Cytometry

Sat Jan 09 00:00:00 UTC 2021

Intranasal Administration of Lentiviral miR-135a Regulates Mast Cell and Allergen-Induced Inflammation by Targeting GATA-3.

PloS one

Deng YQ,Yang YQ,Wang SB,Li F,Liu MZ,Hua QQ,Tao ZZ

Published figure using Gata-3 monoclonal antibody (Product # 53-9966-42) in Flow Cytometry

Tue May 31 00:00:00 UTC 2016

GATA3 suppresses metastasis and modulates the tumour microenvironment by regulating microRNA-29b expression.

Nature cell biology

Chou J,Lin JH,Brenot A,Kim JW,Provot S,Werb Z

Published figure using Gata-3 monoclonal antibody (Product # 53-9966-42) in Immunohistochemistry

Fri Feb 01 00:00:00 UTC 2013

Impaired proteasome function activates GATA3 in T cells and upregulates CTLA-4: relevance for Sézary syndrome.

The Journal of investigative dermatology

Gibson HM,Mishra A,Chan DV,Hake TS,Porcu P,Wong HK

Published figure using Gata-3 monoclonal antibody (Product # 53-9966-42) in Flow Cytometry

Tue Jan 01 00:00:00 UTC 2013

Mesenchymal stem cells alleviate TNBS-induced colitis by modulating inflammatory and autoimmune responses.

World journal of gastroenterology

Chen QQ,Yan L,Wang CZ,Wang WH,Shi H,Su BB,Zeng QH,Du HT,Wan J

Published figure using Gata-3 monoclonal antibody (Product # 53-9966-42) in Western Blot

Wed Aug 07 00:00:00 UTC 2013

Role of CD44 in the differentiation of Th1 and Th2 cells: CD44-deficiency enhances the development of Th2 effectors in response to sheep RBC and chicken ovalbumin.

Journal of immunology (Baltimore, Md. : 1950)

Guan H,Nagarkatti PS,Nagarkatti M

Wed Jul 01 00:00:00 UTC 2009

Bioinformatics

Protein Aliases: GATA binding factor-3; GATA binding protein-3; GATA-binding factor 3; GATA-binding protein 3; MGC2346; MGC5199; MGC5445; Trans-acting T-cell-specific transcription factor GATA-3; Transacting T-cell-specific transcription factor GATA-3

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Gene Aliases: EGK_19445; Gata-3; GATA3; HDR; HDRS; jal

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UniProt ID: (Human) P23771, (Mouse) P23772

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Entrez Gene ID: (Pig) 733631, (Rhesus monkey) 713840, (Human) 2625, (Mouse) 14462

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Function(s)

transcription regulatory region sequence-specific DNA binding RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II core promoter sequence-specific DNA binding core promoter proximal region sequence-specific DNA binding core promoter sequence-specific DNA binding nucleic acid binding transcription factor activity transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional repressor activity, RNA polymerase II core promoter proximal region sequence-specific binding RNA polymerase II transcription factor binding enhancer sequence-specific DNA binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding transcription coactivator activity interleukin-2 receptor binding protein binding transcription factor binding zinc ion binding transcription regulatory region DNA binding protein dimerization activity E-box binding HMG box domain binding sequence-specific DNA binding metal ion binding DNA-binding transcription factor

Process(es)

Performance Guarantee

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Gata-3 Monoclonal Antibody (TWAJ), Alexa Fluor™ 488, eBioscience™

Gata-3 Antibody (53-9966-42) in ICC/IF

Product Details

53-9966-42

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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