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|Tested species reactivity||Yeast|
|Host / Isotype||Rabbit / IgG|
|Immunogen||N terminal domain (amino acid residues 1-300) of Gcn5p protein|
|Storage buffer||whole serum|
|Contains||0.1% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay dependent|
|Western Blot (WB)||1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-4929 detects Gcn5p in budding yeast samples.
PA1-4929 has been successfully used in Western blot and ELISA procedures. By Western blot this antibody detects ~54 kD (Gcn5p) in yeast samples.
PA1-4929 immunogen corresponds to the recombinant protein which was over-expressed in E. coli with a plasmid carrying the N terminal domain (amino acid residues 1-300) of Gcn5p protein
SAGA (Spt-Ada-Gen5 histone acetyltransferase complex) is a histoneacetylase complex which has Gcn5p as catalyst subunit and functions overlapping with the fundamental transcription factor TFIID which has Taf1p as catalyst subunit. However, SAGA and TFIID have different allotment and each accomplishes the important role in the transcription for Housekeeping gene group and Stress Responding gene group.
Also, SAGA is a gigantic protein complex which is composed of Ada protein group (5 kinds), TBP related protein group (4 kinds), TAF protein group (5 kinds that also pertain to TFIID), and other protein groups (>6 kinds). As its molecular function, there is the chemical modification of histone, or the recruitment by direct interaction of transcription regulating factor on DNA, or the control of transcription starting reaction by TBP. Gcn5p that show histoneacetylase activity is one kind of the above-mentioned Ada protein group, and in the case of budding yeast it is composed of 439 amino acid residues.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.