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Geminin/DNA Replication Inhibitor Monoclonal Antibody (CPTC-GMNN-1)

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Geminin/DNA Replication Inhibitor Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry/Immunofluorescence analysis of PFA-fixed HeLa cells using Geminin, DNA Replication Inhibitor Monoclonal Antibody (Product # 51053-MSM1-P1ABX) followed by goat anti-Mouse IgG-CF488 (Green). Membrane is labeled with Phalloidin.

Geminin/DNA Replication Inhibitor Antibody in Peptide array (ARRAY) — Analysis of Protein Array containing more than 19,000 full-length human proteins Geminin, DNA Replication Inhibitor Monoclonal Antibody (Product # 51053-MSM1-P1ABX). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.

Geminin/DNA Replication Inhibitor Antibody — SDS-PAGE Analyis Purified Geminin, DNA Replication Inhibitor Monoclonal Antibody (Product # 51053-MSM1-P1ABX). Confirmation of Purity and Integrity of Antibody.

Product Details

51053-MSM1-P1ABX

Applications	Tested Dilution	Publications
Immunohistochemistry (Paraffin) (IHC (P))	1-2 µg/mL	-
Immunocytochemistry (ICC/IF)	1-2 µg/mL	-
Peptide Array (Array)	Assay-dependent	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Mouse / IgG1, kappa
Class	Monoclonal
Type	Antibody
Clone	CPTC-GMNN-1
Immunogen	Recombinant human full-length protein View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Protein G
Storage buffer	PBS, pH 7.4
Contains	no preservative
Storage conditions	-20°C or -80°C if preferred

Product Specific Information

Immunohistochemistry (PFA fixed): incubate antibody for 30 min RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 degrees C followed by cooling at RT for 20 minutes.

Target Information

GMNN inhibits DNA replication by preventing the incorporation of MCM complex into prereplication complex (pre-RC). It is degraded during the mitotic phase of the cell cycle. Its destruction at the metaphase-anaphase transition permits replication in the succeeding cell cycle.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Geminin; Geminin GMNN; OTTHUMP00000039393; RP3 369A17.3

Gene Aliases: Gem; GMNN; MGORS6

UniProt ID: (Human) O75496

Entrez Gene ID: (Human) 51053

Function(s)

protein complex assembly cell cycle negative regulation of DNA replication organ morphogenesis negative regulation of cell cycle negative regulation of transcription, DNA-templated

Process(es)

transcription corepressor activity protein binding histone deacetylase binding repressing transcription factor binding

Geminin/DNA Replication Inhibitor Monoclonal Antibody (CPTC-GMNN-1)

Product Details

51053-MSM1-P1ABX

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Peptide Array (Array)

Species Reactivity

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Product Specific Information

Target Information

References (0)

Bioinformatics

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