|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||synthetic peptide LRGAPSASRGSYTQQPR-C conjugated to KLH, corresponding to amino acids 1193-1209 of Mouse Gli2|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.02M potassium phosphate, pH 7.2, with 0.15M NaCl|
|Contains||0.01% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||2-20 µg/ml|
|Western Blot (WB)||1:500-1:200|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-28838 detects Gli2 from mouse samples.
PA1-28838 has been successfully used in immunohistochemistry (paraffin tissue), Western blot, and ELISA applications.
The PA1-28838 immunogen is a Synthetic peptide, LRGAPSASRGSYTQQPR-C conjugated to KLH, corresponding to amino acids 1193-1209 of Mouse Gli2.
Store at 4°C short term. Aliquot and store at -20°C or below. Avoid repeated freez/thaw cycles.
This protein belongs to the C2H2-type zinc finger protein subclass of the Gli family. Members of this subclass are characterized as transcription factors that bind DNA through zinc finger motifs. These motifs contain conserved H-C links. Gli family zinc finger proteins are mediators of Sonic hedgehog (Shh) signaling and they are implicated as potent oncogenes in the embryonal carcinoma cell. The protein encoded by this gene localizes to the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. Gli2 is also thought to play a role during embryogenesis. The encoded protein is associated with several phenotypes- Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, preaxial polydactyly type IV, postaxial polydactyly types A1 and B.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.