Immunofluorescent analysis of Gli3 in MCF-7 cells using a Gli3 polyclonal antibody (Product # PA1-29595) and an alpha-Tubulin antibody, both at 5 µg/mL for 1 hr at RT. Secondary antibody was used at a dilution of 1:10,000 for 45 min at room temperature. Antigen retrieval was not required.
|Tested species reactivity||Human, Mouse, Xenopus|
|Host / Isotype||Rabbit / IgG|
|Immunogen||synthetic peptide NEDESPGQTYHRERRNA-C conjugated to KLH, corresponding to amino acids 41-57 of Human GLI3.|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.02M potassium phosphate, pH 7.2, with 0.15M NaCl|
|Contains||0.1% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||0.5 µg/ml-5 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||0.5 µg/ml-5 µg/ml|
|Western Blot (WB)||1:500-1:2000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-29595 detects Gli3 from human samples. Expected to cross react with chicken (100% identity), human (100% identity), mouse, (94% identity), Xenopus laevis (100% identity) due to sequence homology.
PA1-29595 has been successfully used in immunohistochemistry (frozen tissue), Western blot, and ELISA applications.
The PA1-29595 immunogen is Synthetic peptide: NEDESPGQTYHRERRNA-C conjugated to KLH, corresponding to amino acids 41-57 of Human GLI3.
Store at 4°C. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
This gene encodes a protein which belongs to the C2H2-type zinc finger proteins subclass of the Gli family. They are characterized as DNA-binding transcription factors and are mediators of Sonic hedgehog (Shh) signaling. The protein encoded by this gene localizes in the cytoplasm and activates patched Drosophila homolog (PTCH) gene expression. It is also thought to play a role during embryogenesis. Mutations in this gene have been associated with several diseases, including Greig cephalopolysyndactyly syndrome, Pallister-Hall syndrome, preaxial polydactyly type IV, and postaxial polydactyly types A1 and B.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.