Note: You clicked on an external link, which has been disabled in order to keep your shopping session open.
Immunofluorescence analysis of GluR2 was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with GluR2 (6C4) Mouse Monoclonal Antibody (320300) at 2ug/ml in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows a no primary antibody control. The images were captured at 60X magnification.
|Tested species reactivity||Human, Mouse, Non-human primate, Rat|
|Published species reactivity||Rat, Non-human primate, Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||Fusion protein containing amino acids 175-430 from the N-terminal region of GluR2.|
|Storage buffer||PBS, pH 7.4, with 50% glycerol, 1% BSA|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||0.5-5 ug/ml|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunocytochemistry (ICC)||2 µg/ml|
|Immunofluorescence (IF)||2 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20-1:100|
|Western Blot (WB)||2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to a family of glutamate receptors that are sensitive to alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA), and function as ligand-activated cation channels. These channels are assembled from 4 related subunits, Gria1-4. The subunit encoded by this gene (Gria2) is subject to RNA editing (Q/R and R/G), which is thought to render the channels impermeable to Ca(2+), and to affect the kinetic aspects of these channels in rat brain. Alternative splicing, resulting in transcript variants encoding different isoforms (flip and flop), has been noted for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Rapid neurogenesis through transcriptional activation in human stem cells.
32-0300 was used in immunocytochemistry to describe a method to rapidly generate neurons from human-induced pluripotent stem cells to elucidate the underlying regulatory programs
|Busskamp V,Lewis NE,Guye P,Ng AH,Shipman SL,Byrne SM,Sanjana NE,Murn J,Li Y,Li S,Stadler M,Weiss R,Church GM||Molecular systems biology (10:null)||2014|
Redistribution of ionotropic glutamate receptors detected by laser microdissection of the rat dentate gyrus 48 h following LTP induction in vivo.
32-0300 was used in western blot to study the effect of LTP induction on the distribution of ionotropic glutamate receptors in vivo
|Kennard JT,Guévremont D,Mason-Parker SE,Abraham WC,Williams JM||PloS one (9:null)||2014|
Rapid visual stimulation increases extrasynaptic glutamate receptor expression but not visual-evoked potentials in the adult rat primary visual cortex.
32-0300 was used in western blot to study the visual-evoked potential in the primary visual cortex of adult rats.
|Eckert MJ,Guévremont D,Williams JM,Abraham WC||The European journal of neuroscience (37:400)||2013|
|Rat||Not Cited||Increased expression, but not postsynaptic localisation, of ionotropic glutamate receptors during the late-phase of long-term potentiation in the dentate gyrus in vivo.||Kennard JT,Guévremont D,Mason-Parker SE,Abraham WC,Williams JM||Neuropharmacology (56:66)||2009|
|Rat||Not Cited||Differential trafficking of AMPA and NMDA receptors during long-term potentiation in awake adult animals.||Williams JM,Guévremont D,Mason-Parker SE,Luxmanan C,Tate WP,Abraham WC||The Journal of neuroscience : the official journal of the Society for Neuroscience (27:14171)||2007|
|Rat||Not Cited||A molecular mechanism for stabilization of learning-induced synaptic modifications.||Quinlan EM,Lebel D,Brosh I,Barkai E||Neuron (41:185)||2004|
|Non-human primate||Not Cited||Glutamate receptor subunit GluR2 and NMDAR1 immunoreactivity in the retina of macaque monkeys with experimental glaucoma does not identify vulnerable neurons.||Hof PR,Lee PY,Yeung G,Wang RF,Podos SM,Morrison JH||Experimental neurology (153:234)||1998|
|Human||Not Cited||Structural determinants of ion flow through recombinant glutamate receptor channels.||Verdoorn TA,Burnashev N,Monyer H,Seeburg PH,Sakmann B||Science (New York, N.Y.) (252:1715)||1991|
AMPA 2; AMPA selective glutamate receptor; AMPA-selective glutamate receptor 2; gluR-2; gluR-B; gluR-K2; GLUR2; GLURB; glutamate receptor; glutamate receptor 2; glutamate receptor B; glutamate receptor B flip isoform; Glutamate receptor ionotropic; glutamate receptor ionotropic, AMPA 2; glutamate receptor, ionotropic, AMPA 2; GRIA2; HBGR2; ionotropic
GluA2; Glur-2; GluR-B; GluR-K2; GLUR2; GLURB; GRIA2; HBGR2