Invitrogen

GluR2 Monoclonal Antibody (6C4)

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

10 Published Figures

28 References

View all (26) GluR2 antibodies

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Protocols

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GluR2 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of GluR2 was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with GluR2 (6C4) Mouse Monoclonal Antibody (Product # 32-0300) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows a no primary antibody control. The images were captured at 60X magnification.

GluR2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of GLUR2 showing staining in the membrane and weak staining in the cytoplasm of paraffin-embedded mouse brain tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a GLUR2 Mouse Monoclonal Antibody (Product # 32-0300) diluted in 3% BSA-PBS at a dilution of 1:100 for 1 hour at 37ºC in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

GluR2 Antibody in Western Blot (WB) — Western blot was performed using Anti-GluR2 Monoclonal Antibody (6C4) (Product # 32-0300) and a 100kDa band corresponding to GluR2 was observed across tissues tested except Mouse Skeletal Muscle and Rat Skeletal Muscle. Tissue extracts (30 µg lysate) of Mouse Brain (Lane 1), Mouse Cerebellum (Lane 2), Rat Brain (Lane 3), Mouse Skeletal Muscle (Lane 4) and Rat Skeletal Muscle (Lane 5) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (2µg/ml) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005). An uncharacterized band (*) was also observed at ~60kDa. A ~25kDa band corresponding to circulating tissue IgG was observed in the Mouse tissue lysates..

GluR2 Antibody in Western Blot, Immunoprecipitation (WB, IP) — Published figure using GluR2 monoclonal antibody (Product # 32-0300) in Western Blot and Immunoprecipitation

GluR2 Antibody — Figure 7 Lithium treatment significantly increases surface GluR1/GluR2 in the hippocampus, but not frontal cortex in vivo Mice were treated with lithium or control chow for 1.5 days. Following treatment, mice were decapitated and brains were rapidly removed. Hippocampal (HP) and frontal cortex (FC) tissues were isolated and the BS3 cross-linking procedure was performed to identify surface GluR1/GluR2 tetramers. Western blot analyses were performed with anti-GluR1 and anti-GluR2 antibodies. Representative images for cross-linked and intracellular GluR1 and GluR2 by western blot analysis (a, b). Densitometry analysis: Lithium significantly enhanced surface cross-linked GluR1 and GluR2 in hippocampus, but not in prefrontal cortex vivo. N=6 mice per group (b-e). * p <0.05 {TM}

Product Details

32-0300

Applications	Tested Dilution	Publications
Western Blot (WB)	2 µg/mL	View 15 publications 15 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:100	-
Immunocytochemistry (ICC/IF)	2 µg/mL	View 5 publications 5 publications
ELISA (ELISA)	0.5-5 µg/mL	-
Immunohistochemistry (IHC)	-	View 5 publications 5 publications
Miscellaneous PubMed (Misc)	-	View 2 publications 2 publications
Immunoprecipitation (IP)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Non-human primate, Rat
Published species	Human, Mouse, Non-human primate, Rat
Host/Isotype	Mouse / IgG1, kappa
Class	Monoclonal
Type	Antibody
Clone	6C4
Immunogen	Fusion protein containing amino acids 175-430 from the N-terminal region of GluR2. View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.5 mg/mL
Purification	Protein A
Storage buffer	PBS, pH 7.4
Contains	0.1% sodium azide
Storage conditions	-20°C
RRID	AB_2533058

Target Information

The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by alpha-amino-3-hydroxy-5 -methyl-4-isoxalone propionic acid (AMPA) are known as AMPA receptors (AMPAR). The AMPAR are comprised of four distinct Glutamate Receptor Subunits designated (GluR1-4) and they play key roles in virtually all excitatory neurotransmission in the brain. The GluR2 subunit is thought to play a key role in forms of synaptic plasticity such as LTD.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Prickle promotes the formation and maintenance of glutamatergic synapses by stabilizing the intercellular planar cell polarity complex.

Science advances

Ban Y,Yu T,Feng B,Lorenz C,Wang X,Baker C,Zou Y

Published figure using GluR2 monoclonal antibody (Product # 32-0300) in Western Blot

Fri Oct 08 00:00:00 EDT 2021

Role of Satb1 and Satb2 Transcription Factors in the Glutamate Receptors Expression and Ca²⁺ Signaling in the Cortical Neurons In Vitro.

International journal of molecular sciences

Turovsky EA,Turovskaya MV,Fedotova EI,Babaev AA,Tarabykin VS,Varlamova EG

Published figure using GluR2 monoclonal antibody (Product # 32-0300) in Immunocytochemistry

Mon May 31 00:00:00 EDT 2021

Role of Satb1 and Satb2 Transcription Factors in the Glutamate Receptors Expression and Ca²⁺ Signaling in the Cortical Neurons In Vitro.

International journal of molecular sciences

Turovsky EA,Turovskaya MV,Fedotova EI,Babaev AA,Tarabykin VS,Varlamova EG

Published figure using GluR2 monoclonal antibody (Product # 32-0300) in Immunocytochemistry

Mon May 31 00:00:00 EDT 2021

Evidence that Brain-Reactive Autoantibodies Contribute to Chronic Neuronal Internalization of Exogenous Amyloid-β1-42 and Key Cell Surface Proteins During Alzheimer's Disease Pathogenesis.

Journal of Alzheimer's disease : JAD

Goldwaser EL,Acharya NK,Wu H,Godsey GA,Sarkar A,DeMarshall CA,Kosciuk MC,Nagele RG

32-0300 was used in Immunohistochemistry to examine consequences of increased blood-brain barrier (BBB) permeability on the development of Alzheimer's disease (AD)-related pathology by tracking selected leaked plasma components and their interactions with neurons in vivo and in vitro.

Thu May 13 00:00:00 EDT 2021

Trans-synaptic assemblies link synaptic vesicles and neuroreceptors.

Science advances

Martinez-Sanchez A,Laugks U,Kochovski Z,Papantoniou C,Zinzula L,Baumeister W,Lučić V

32-0300 was used in Sample Preparation to investigate if synaptic function is carried by precisely organised trans-synaptic units.

Mon Mar 01 00:00:00 EST 2021

Sex differences in the role of atypical PKC within the basolateral nucleus of the amygdala in a mouse hyperalgesic priming model.

Neurobiology of pain (Cambridge, Mass.)

Baptista-de-Souza D,Tavares-Ferreira D,Megat S,Sankaranarayanan I,Shiers S,Flores CM,Ghosh S,Luiz Nunes-de-Souza R,Canto-de-Souza A,Price TJ

32-0300 was used in Immunohistochemistry to elucidate the molecular mechanisms in the basolateral nucleus of the amygdala (BLA) that modulate hyperalgesic priming, a pain plasticity model, in males and females.

Mon Sep 28 00:00:00 EDT 2020

Distinct Cell Surface Expression Patterns of N-Glycosylation Site Mutants of AMPA-Type Glutamate Receptor under the Homo-Oligomeric Expression Conditions.

International journal of molecular sciences

Morise J,Yamamoto S,Midorikawa R,Takamiya K,Nonaka M,Takematsu H,Oka S

32-0300 was used in Immunocytochemistry-immunoflourescence to suggest that these N-glycans have distinct roles in the ER-exit of GluA1 and GluA2 homo-oligomers; N-glycan at GluA1N63 is a prerequisite for GluA1 ER-exit, whereas N-glycans at GluA1N363 and GluA2N370 control the ER-exit rate.

Sun Jul 19 00:00:00 EDT 2020

Glutamate Receptor Trafficking and Protein Synthesis Mediate the Facilitation of LTP by Secreted Amyloid Precursor Protein-Alpha.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Mockett BG,Guévremont D,Elder MK,Parfitt KD,Peppercorn K,Morrissey J,Singh A,Hintz TJ,Kochen L,Tom Dieck S,Schuman E,Tate WP,Williams JM,Abraham WC

32-0300 was used in Western Blotting to demonstrate that exposure to secreted amyloid precursor protein-alpha converts short-lasting long-term potentiation into protein-synthesis-dependent late LTP in hippocampal slices from male rats.

Wed Apr 24 00:00:00 EDT 2019

The Temporal Dynamics of Arc Expression Regulate Cognitive Flexibility.

Neuron

Wall MJ,Collins DR,Chery SL,Allen ZD,Pastuzyn ED,George AJ,Nikolova VD,Moy SS,Philpot BD,Shepherd JD,Müller J,Ehlers MD,Mabb AM,Corrêa SAL

32-0300 was used in Western Blotting to study how the precise temporal control of activity-dependent molecules, such as Arc, regulates synaptic plasticity and is crucial for cognition.

Wed Jun 27 00:00:00 EDT 2018

NMDA receptors are selectively partitioned into complexes and supercomplexes during synapse maturation.

Nature communications

Frank RA,Komiyama NH,Ryan TJ,Zhu F,O'Dell TJ,Grant SG

32-0300 was used in western blot to analyze selective partitioning into complexes and supercomplexes during synapse maturation by NMDA receptors

Wed Apr 27 00:00:00 EDT 2016

Mice with Shank3 Mutations Associated with ASD and Schizophrenia Display Both Shared and Distinct Defects.

Neuron

Zhou Y,Kaiser T,Monteiro P,Zhang X,Van der Goes MS,Wang D,Barak B,Zeng M,Li C,Lu C,Wells M,Amaya A,Nguyen S,Lewis M,Sanjana N,Zhou Y,Zhang M,Zhang F,Fu Z,Feng G

Published figure using GluR2 monoclonal antibody (Product # 32-0300) in Western Blot

Wed Jan 06 00:00:00 EST 2016

Cocaine-induced plasticity in the cerebellum of sensitised mice.

Psychopharmacology

Vazquez-Sanroman D,Carbo-Gas M,Leto K,Cerezo-Garcia M,Gil-Miravet I,Sanchis-Segura C,Carulli D,Rossi F,Miquel M

32-0300 was used in immunohistochemistry to study sensitized mice and cocaine-induced plasticity in the cerebellum

Tue Dec 01 00:00:00 EST 2015

L-Stepholidine rescues memory deficit and synaptic plasticity in models of Alzheimer's disease via activating dopamine D1 receptor/PKA signaling pathway.

Cell death & disease

Hao JR,Sun N,Lei L,Li XY,Yao B,Sun K,Hu R,Zhang X,Shi XD,Gao C

32-0300 was used in western blot to utilize models of Alzheimer's disease via activating dopamine D1 receptor/PKA signaling pathway where L-Stepholidine rescues memory deficit and synaptic plasticity

Thu Nov 05 00:00:00 EST 2015

Redistribution of ionotropic glutamate receptors detected by laser microdissection of the rat dentate gyrus 48 h following LTP induction in vivo.

PloS one

Kennard JT,Guévremont D,Mason-Parker SE,Abraham WC,Williams JM

32-0300 was used in western blot to study the effect of LTP induction on the distribution of ionotropic glutamate receptors in vivo

Fri Jan 30 00:00:00 EST 2015

Rapid neurogenesis through transcriptional activation in human stem cells.

Molecular systems biology

Busskamp V,Lewis NE,Guye P,Ng AH,Shipman SL,Byrne SM,Sanjana NE,Murn J,Li Y,Li S,Stadler M,Weiss R,Church GM

32-0300 was used in immunocytochemistry to describe a method to rapidly generate neurons from human-induced pluripotent stem cells to elucidate the underlying regulatory programs

Mon Nov 17 00:00:00 EST 2014

GluA2 mRNA distribution and regulation by miR-124 in hippocampal neurons.

Molecular and cellular neurosciences

Ho VM,Dallalzadeh LO,Karathanasis N,Keles MF,Vangala S,Grogan T,Poirazi P,Martin KC

Published figure using GluR2 monoclonal antibody (Product # 32-0300) in Western Blot

Tue Jul 01 00:00:00 EDT 2014

GluA2 mRNA distribution and regulation by miR-124 in hippocampal neurons.

Molecular and cellular neurosciences

Ho VM,Dallalzadeh LO,Karathanasis N,Keles MF,Vangala S,Grogan T,Poirazi P,Martin KC

Published figure using GluR2 monoclonal antibody (Product # 32-0300) in Western Blot

Tue Jul 01 00:00:00 EDT 2014

GluA2 mRNA distribution and regulation by miR-124 in hippocampal neurons.

Molecular and cellular neurosciences

Ho VM,Dallalzadeh LO,Karathanasis N,Keles MF,Vangala S,Grogan T,Poirazi P,Martin KC

Published figure using GluR2 monoclonal antibody (Product # 32-0300) in Western Blot

Tue Jul 01 00:00:00 EDT 2014

Rapid visual stimulation increases extrasynaptic glutamate receptor expression but not visual-evoked potentials in the adult rat primary visual cortex.

The European journal of neuroscience

Eckert MJ,Guévremont D,Williams JM,Abraham WC

32-0300 was used in western blot to study the visual-evoked potential in the primary visual cortex of adult rats.

Fri Feb 01 00:00:00 EST 2013

Increased expression, but not postsynaptic localisation, of ionotropic glutamate receptors during the late-phase of long-term potentiation in the dentate gyrus in vivo.

Neuropharmacology

Kennard JT,Guévremont D,Mason-Parker SE,Abraham WC,Williams JM

Thu Jan 01 00:00:00 EST 2009

Involvement of AMPA receptors in the antidepressant-like effects of lithium in the mouse tail suspension test and forced swim test.

Neuropharmacology

Gould TD,O'Donnell KC,Dow ER,Du J,Chen G,Manji HK

Published figure using GluR2 monoclonal antibody (Product # 32-0300) in Western Blot

Sat Mar 01 00:00:00 EST 2008

Differential trafficking of AMPA and NMDA receptors during long-term potentiation in awake adult animals.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Williams JM,Guévremont D,Mason-Parker SE,Luxmanan C,Tate WP,Abraham WC

Wed Dec 19 00:00:00 EST 2007

Learning-induced glutamate receptor phosphorylation resembles that induced by long term potentiation.

The Journal of biological chemistry

Shukla K,Kim J,Blundell J,Powell CM

Published figure using GluR2 monoclonal antibody (Product # 32-0300) in Immunoprecipitation

Fri Jun 22 00:00:00 EDT 2007

Learning-induced glutamate receptor phosphorylation resembles that induced by long term potentiation.

The Journal of biological chemistry

Shukla K,Kim J,Blundell J,Powell CM

Published figure using GluR2 monoclonal antibody (Product # 32-0300) in Immunoprecipitation

Fri Jun 22 00:00:00 EDT 2007

A molecular mechanism for stabilization of learning-induced synaptic modifications.

Neuron

Quinlan EM,Lebel D,Brosh I,Barkai E

Thu Jan 22 00:00:00 EST 2004

Glutamate receptor subunit 3 (GluR3) immunoreactivity delineates a subpopulation of parvalbumin-containing interneurons in the rat hippocampus.

The Journal of comparative neurology

Moga DE,Janssen WG,Vissavajjhala P,Czelusniak SM,Moran TM,Hof PR,Morrison JH

Mon Jul 14 00:00:00 EDT 2003

Glutamate receptor subunit 3 (GluR3) immunoreactivity delineates a subpopulation of parvalbumin-containing interneurons in the rat hippocampus.

The Journal of comparative neurology

Moga DE,Janssen WG,Vissavajjhala P,Czelusniak SM,Moran TM,Hof PR,Morrison JH

Mon Jul 14 00:00:00 EDT 2003

Glutamate receptor subunit GluR2 and NMDAR1 immunoreactivity in the retina of macaque monkeys with experimental glaucoma does not identify vulnerable neurons.

Experimental neurology

Hof PR,Lee PY,Yeung G,Wang RF,Podos SM,Morrison JH

Thu Oct 01 00:00:00 EDT 1998

Structural determinants of ion flow through recombinant glutamate receptor channels.

Science (New York, N.Y.)

Verdoorn TA,Burnashev N,Monyer H,Seeburg PH,Sakmann B

Fri Jun 21 00:00:00 EDT 1991

Bioinformatics

Protein Aliases: AMPA selective glutamate receptor; AMPA-selective glutamate receptor 2; GluA2; GluR-2; GluR-B; GluR-K2; glutamate receptor; Glutamate receptor 2; glutamate receptor B; glutamate receptor B flip isoform; Glutamate receptor ionotropic, AMPA 2; glutamate receptor, ionotropic, AMPA 2; OTTHUMP00000165324; OTTHUMP00000220942; OTTHUMP00000220943

Gene Aliases: GluA2; Glur-2; GluR-B; GluR-K2; GLUR2; GLURB; GRIA2; HBGR2

UniProt ID: (Human) P42262, (Rat) P19491

Entrez Gene ID: (Human) 2891, (Mouse) 14800, (Rat) 29627

Molecular Function: transmembrane signal receptor