Invitrogen

GluR2 Monoclonal Antibody (7G6)

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This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

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Datasheet

Protocols

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GluR2 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of GluR2 Monoclonal Antibody (7G6) was performed using 70% confluent log phase Neuro-2a cells. The cells were fixed with 4% Paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 10 minutes at room temperature. The cells were labeled with GluR2 Monoclonal Antibody (Product # MA5-17084) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175, 1:2000 dilution) for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing membrane localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification..

GluR2 Antibody in Western Blot (WB) — Western blot was performed using Anti-GluR2 Monoclonal Antibody (7G6) (Product # MA5-17084) and a 100kDa band corresponding to GluR2 was observed across all cell lines and tissues tested except Mouse Heart and Rat Heart which is reported to be negative. Membrane enriched cell extracts (30 µg lysate) of Neuro2A (Lane 1), Mouse Brain (Lane 2), Mouse Cerebellum (Lane 3), Rat Brain (Lane 4), Mouse Heart (Lane 5) and Rat Heart (Lane 6) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005). A ~25kDa band corresponding to circulating tissue IgG was observed in case of Mouse tissue lysates..

GluR2 Antibody in Flow Cytometry (Flow) — Flow cytometric analysis of SK-N-SH cells using GRIA2 monoclonal antibody (Product # MA5-17084) (green) and negative control (red).

GluR2 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cells and tissues tested owing to their inherent genetic constitution. Relative expression of GluR2 was observed in Neuro2A, Mouse Brain, Mouse Cerebellum and Rat Brain in comparison to Mouse Heart and Rat Heart by using Anti-GluR2 Monoclonal Antibody (7G6) (Product # MA5-17084).. {RE}

Product Details

MA5-17084

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	-
Immunocytochemistry (ICC/IF)	1:100	-
Flow Cytometry (Flow)	1:200 - 1:400	-
ELISA (ELISA)	1:10,000	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	7G6
Immunogen	Purified recombinant fragment of human GRIA2 (amino acids: 652-807) expressed in E. Coli. View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Protein G
Storage buffer	PBS
Contains	0.05% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2538555

Product Specific Information

MA5-17084 targets GRIA2 in FACS, pep-ELISA, and WB applications and shows reactivity with Human samples.

The MA5-17084 immunogen is purified recombinant fragment of human GRIA2 (amino acids: 652-807) expressed in E. Coli.

MA5-17084 detects GRIA2 which has a predicted molecular weight of approximately 98.8kDa.

Target Information

The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by alpha-amino-3-hydroxy-5 -methyl-4-isoxalone propionic acid (AMPA) are known as AMPA receptors (AMPAR). The AMPAR are comprised of four distinct Glutamate Receptor Subunits designated (GluR1-4) and they play key roles in virtually all excitatory neurotransmission in the brain. The GluR2 subunit is thought to play a key role in forms of synaptic plasticity such as LTD.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: AMPA selective glutamate receptor; AMPA-selective glutamate receptor 2; GluA2; GluR-2; GluR-B; GluR-K2; glutamate receptor; Glutamate receptor 2; glutamate receptor B; glutamate receptor B flip isoform; Glutamate receptor ionotropic, AMPA 2; glutamate receptor, ionotropic, AMPA 2; OTTHUMP00000165324; OTTHUMP00000220942; OTTHUMP00000220943

Gene Aliases: GluA2; Glur-2; GluR-B; GluR-K2; GLUR2; GLURB; GRIA2; HBGR2

UniProt ID: (Human) P42262, (Rat) P19491

Entrez Gene ID: (Human) 2891, (Mouse) 14800, (Rat) 29627

Molecular Function: transmembrane signal receptor