Invitrogen

GluR3 Monoclonal Antibody (3B3)

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

4 Published Figures

8 References

View all (14) GluR3 antibodies

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GluR3 Antibody in Western Blot (WB) — Figure 4 Activation of D1/PKA rescues the decreased GluA1-containing AMPA receptor surface expression induced by ADDLs in cultured hippocampal neurons. ( a - c ) Surface expression of GluA1 (S-GluA1) and GluA2 (S-GluA2) were significantly decreased after treating with ADDLs (500 nM) for 3-24 h, whereas surface expression of GluA3 (S-GluA3) was not changed. Total expression of GluA1-3 (T-GluA) were not changed. (GluA1: n =5 in each group, GluA2: n =4 in each group, GluA3: n =4 in each group). ( d ) Phosphorylated levels of GluA1 at Serine 845 (pS845) and Serine 831 (pS831) were decreased after treating with ADDLs (pS845: n =9 in each group, pS831: n =9 in each group). ( e ) Pretreatment with PKA activator Sp-cAMPS (10 muM) rescued the decreased pS845 and surface expression of GluA1 induced by ADDLs, whereas the PKA inhibitor Rp-cAMPS (10 muM) reversed these effects. Sp-cAMPS or Rp-cAMPS was added 10 min before ADDLs ( n =4 in each group). ( f ) Pretreatment with D1-type receptor agonist SKF 81297 (SKF, 3 muM) rescued the decreased pS845 and surface expression of GluA1 induced by ADDLs, whereas the antagonist SCH 23390 (SCH, 10 muM) reversed these effects. SKF 81297 or SCH 23390 was added 15 min before ADDLs ( n =6 in each group). * P <0.05, ** P <0.01, *** P <0.001 versus corresponding control group (Con); # P <0.05, ## P <0.01, ### P <0.001 versus corresponding ADDL group. Data are represented as mean+-S.E.M

GluR3 Antibody — Figure 4. Open in a separate window A , B , Changes in AMPA receptor subunit expression of the AMPA receptor subunits GluR-A, GluR-B, GluR-C, and GluR-D in hippocampus ( A ) and cerebellum ( B ) of wild-type (WT) and GluR-C knock-out (GluR-C -/-) mice from P2 to P90. Total proteins of the hippocampus and the cerebellum were isolated, and for each sample, 10 mug of protein was separated on a 7.5% SDS-PAGE and transferred on nitrocellulose. The glutamate receptor subunits were visualized by selective antibodies and monitored by autoradiography. The alpha-subunit of the Ca 2+ /calmodulin-dependent protein kinase II (alpha-CaMKII) and betaIII-tubulin (betaIII-Tub.) was used as a positive control for developmental controlled gene expression in hippocampus and cerebellum, respectively. Loading was controlled by beta-actin. The experiment was repeated with at least three sets of mice. One representative example for each subunit is given. {KO}

Product Details

32-0400

Applications	Tested Dilution	Publications
Western Blot (WB)	5 µg/mL	View 7 publications 7 publications
Immunocytochemistry (ICC/IF)	1-3 µg/mL	View 1 publication 1 publication

Product Specifications
Species Reactivity	Non-human primate, Rat, Human
Published species	Mouse, Rat, Tag
Host/Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Clone	3B3
Immunogen	Fusion protein encompassing the N-terminal region of rat GluR3. View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.5 mg/mL
Purification	Protein A
Storage buffer	PBS, pH 7.4
Contains	0.1% sodium azide
Storage conditions	-20°C
RRID	AB_2533059

Target Information

Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes composed of multiple subunits, arranged to form ligand-gated ion channels. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. The subunit encoded by this gene belongs to a family of AMPA (alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate)-sensitive glutamate receptors, and is subject to RNA editing (AGA->GGA; R->G). Alternative splicing at this locus results in different isoforms, which may vary in their signal transduction properties.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Hippocampal AMPA receptor assemblies and mechanism of allosteric inhibition.

Nature

Yu J,Rao P,Clark S,Mitra J,Ha T,Gouaux E

32-0400 was used in Western Blot to demonstrate how the receptor-TARP-γ8 stoichiometry explains the mechanism of and submaximal inhibition by a clinically relevant, brain-region-specific allosteric inhibitor.

Tue Jun 01 00:00:00 EDT 2021

Cognitive aging is associated with redistribution of synaptic weights in the hippocampus.

Proceedings of the National Academy of Sciences of the United States of America

Buss EW,Corbett NJ,Roberts JG,Ybarra N,Musial TF,Simkin D,Molina-Campos E,Oh KJ,Nielsen LL,Ayala GD,Mullen SA,Farooqi AK,D'Souza GX,Hill CL,Bean LA,Rogalsky AE,Russo ML,Curlik DM,Antion MD,Weiss C,Chetkovich DM,Oh MM,Disterhoft JF,Nicholson DA

32-0400 was used in Western Blot to show that the synaptic architecture of hippocampal regions CA1 and CA3 is maintained in a young adult-like state in aged rats that performed comparably to their young adult counterparts in both trace eyeblink conditioning and Morris water maze learning.

Tue Feb 23 00:00:00 EST 2021

L-Stepholidine rescues memory deficit and synaptic plasticity in models of Alzheimer's disease via activating dopamine D1 receptor/PKA signaling pathway.

Cell death & disease

Hao JR,Sun N,Lei L,Li XY,Yao B,Sun K,Hu R,Zhang X,Shi XD,Gao C

32-0400 was used in western blot to utilize models of Alzheimer's disease via activating dopamine D1 receptor/PKA signaling pathway where L-Stepholidine rescues memory deficit and synaptic plasticity

Thu Nov 05 00:00:00 EST 2015

Differential trafficking of AMPA and NMDA receptors during long-term potentiation in awake adult animals.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Williams JM,Guévremont D,Mason-Parker SE,Luxmanan C,Tate WP,Abraham WC

Wed Dec 19 00:00:00 EST 2007

Involvement of the AMPA receptor GluR-C subunit in alcohol-seeking behavior and relapse.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Sanchis-Segura C,Borchardt T,Vengeliene V,Zghoul T,Bachteler D,Gass P,Sprengel R,Spanagel R

Wed Jan 25 00:00:00 EST 2006

Involvement of the AMPA receptor GluR-C subunit in alcohol-seeking behavior and relapse.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Sanchis-Segura C,Borchardt T,Vengeliene V,Zghoul T,Bachteler D,Gass P,Sprengel R,Spanagel R

Wed Jan 25 00:00:00 EST 2006

Involvement of the AMPA receptor GluR-C subunit in alcohol-seeking behavior and relapse.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Sanchis-Segura C,Borchardt T,Vengeliene V,Zghoul T,Bachteler D,Gass P,Sprengel R,Spanagel R

Wed Jan 25 00:00:00 EST 2006

A juvenile form of postsynaptic hippocampal long-term potentiation in mice deficient for the AMPA receptor subunit GluR-A.

The Journal of physiology

Jensen V,Kaiser KM,Borchardt T,Adelmann G,Rozov A,Burnashev N,Brix C,Frotscher M,Andersen P,Hvalby Ø,Sakmann B,Seeburg PH,Sprengel R

Mon Dec 15 00:00:00 EST 2003

Glutamate receptor subunit 3 (GluR3) immunoreactivity delineates a subpopulation of parvalbumin-containing interneurons in the rat hippocampus.

The Journal of comparative neurology

Moga DE,Janssen WG,Vissavajjhala P,Czelusniak SM,Moran TM,Hof PR,Morrison JH

Mon Jul 14 00:00:00 EDT 2003

Glutamate receptor subunit 3 (GluR3) immunoreactivity delineates a subpopulation of parvalbumin-containing interneurons in the rat hippocampus.

The Journal of comparative neurology

Moga DE,Janssen WG,Vissavajjhala P,Czelusniak SM,Moran TM,Hof PR,Morrison JH

Mon Jul 14 00:00:00 EDT 2003

Bioinformatics

Protein Aliases: AMPA-selective glutamate receptor 3; dJ1171F9.1; GluA3; GluR-3; GluR-C; GluR-K3; glutamate receptor; Glutamate receptor 3; glutamate receptor C; Glutamate receptor ionotropic, AMPA 3; glutamate receptor subunit 3; glutamate receptor, ionotrophic, AMPA 3; glutamate receptor, ionotropic, AMPA 3; glutamate receptor, ionotropic, AMPA3 (alpha 3); OTTHUMP00000024262; RP11-349N19.3

Gene Aliases: GluA3; GluR-3; GLUR-C; GLUR-K3; GLUR3; GLURC; GRIA3; MRX94

UniProt ID: (Human) P42263, (Rat) P19492

Entrez Gene ID: (Human) 2892, (Rat) 29628

Molecular Function: transmembrane signal receptor