|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues Y D R R L A Q H K S E I E of mGluR5.|
|Purification||Antigen affinity chromatography|
|Storage buffer||1% BSA|
|Contains||0.1% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 1 publications below|
PA1-24637 detects mGluR5 from mouse and rat samples. PA1-24637 is expected to cross react with human (100% conserved) and chicken (90% conserved) due to sequence homology.
PA1-24637 has been successfully used in Western blot procedures.
The PA1-24637 immunogen is a synthetic peptide corresponding to residues Y D R R L A Q H K S E I E of mGluR5.
Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. This gene product belongs to the kainate family of glutamate receptors, which are composed of four subunits and function as ligand-activated ion channels. The subunit encoded by this gene is subject to RNA editing (CAG->CGG; Q->R) within the second transmembrane domain, which is thought to alter the properties of ion flow. Alternative splicing, resulting in transcript variants encoding different isoforms, has been noted for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Cocaine-induced behavioral sensitization decreases the expression of endocannabinoid signaling-related proteins in the mouse hippocampus.
PA1-24637 was used in western blot to determine if the hippocampal gene/protein expression of relevant glutamate signaling components, cannabinoid type 1 receptor, and endocannabinoid metabolic enzymes were altered following acute and/or repeated cocaine administration
|Blanco E,Galeano P,Palomino A,Pavón FJ,Rivera P,Serrano A,Alen F,Rubio L,Vargas A,Castilla-Ortega E,Decara J,Bilbao A,de Fonseca FR,Suárez J||European neuropsychopharmacology : the journal of the European College of Neuropsychopharmacology (26:477)||2016|