Western blot analysis of Glucagon in whole cell extracts from mouse small intestine using a Glucagon recombinant rabbit monoclonal antibody (Product # 701296) at a dilution of 2ug/ml. Samples were detected using chemiluminescence (ECL). Results show a band at ~21kDa.
|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Peptide corresponding to amino acids 98–118 of human GLP1|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 1 month. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Western Blot (WB)||2-3 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with rat, non-human primate and rabbit based on sequence homology.
ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression vectors, produced on a large scale, and purified with Protein A.
ABfinity™ monoclonal antibodies resemble rabbit monoclonals isolated from serum or produced by hybridomas, but demonstrate greater specificity and sensitivity. Because ABfinity™ recombinant antibodies are derived from cloned DNA sequences of the heavy and light antibody chains, they are not susceptible to cell-line drift or lot-to-lot variation, thus allowing for peak specificity and performance.
Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.
Glucagon is a peptide hormone secreted by alpha cells in the Islet of Langerhans of the pancreas. The peptide is synthesized as a 180 amino acid precursor which is converted to glucagon by prohormone convertase 2 (PC2). The primary role of glucagon is in gluconeogenesis and glycogenolysis. It stimulates the release of glucose by converting glycogen in liver, and also maintains glucose homeostasis in coordination with insulin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.