|ChIP assay (ChIP)||Assay dependent|
|Gel Shift (GS)||Assay dependent|
|Immunocytochemistry (ICC)||Assay dependent|
|Immunohistochemistry (Frozen) (IHC (F))||1 µg/ml|
|Immunoprecipitation (IP)||5 µg/ml|
|Western Blot (WB)||5 µg/ml|
|Immunocytochemistry (ICC)||See 3 publications below|
|ChIP assay (ChIP)||See 3 publications below|
|Western Blot (WB)||See 14 publications below|
|Immunohistochemistry (IHC)||See 3 publications below|
|Flow Cytometry (Flow)||See 1 publications below|
|Gel Shift (GS)||See 2 publications below|
|Tested Species reactivity||Human, Mouse, Rat|
|Published species reactivity||Rat , Non-human primate , Mouse Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues C(245) K P L I L P D T K P K I K D(259) of human GR.|
|Purification||Antigen affinity chromatography|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
PA1-512 detects glucocorticoid receptor (GR) from human, mouse and rat tissues.
PA1-512 has been successfully used in Western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunofluorescence, ChIP and gel shift procedures. By Western blot, this antibody detects an ~97 kDa protein representing GR as well as two other unidentified proteins at ~135 to ~145 kDa from rat liver extract. These cross reacting proteins are not believed to be GR or GR precursors as they do not bind [3H]dexamethasone mesylate. Immunohistochemical staining of GR in rat hippocampus using PA1-512 results in staining primarily in the nucleus. PA1-512 does not disrupt receptor-DNA interactions.
The PA1-512 immunogen is a synthetic peptide corresponding to residues C(245) K P L I L P D T K P K I K D(259) of human GR. The PA1-512 immunizing peptide (Cat. # PEP-002) is available for use in for neutralization and control experiments.
Reconstitute with PBS.
Steroid receptors are ligand-dependent, intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. Glucocorticoids are a family of steroids necessary for the regulation of energy metabolism and the immune and inflammatory responses. These compounds exert their effect through their interaction with the glucocoticoid receptor (GR) and that complex's subsequent association with DNA. All normal mammalian tissues examined to date have been shown to contain glucocorticoid receptor.
The corresponding gene for the glucocorticoid receptor is NR3C1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: GCCR; GCR; glucocorticoid nuclear receptor variant 1; Glucocorticoid receptor; glucocorticoid receptor 1; Glucocorticoid receptor lymphocyte; GR; GRL; Grl1; NR3C1; Nuclear receptor subfamily 3 group C member 1; nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)
Gene Aliases: GCCR; GCR; GCRST; GR; GRL; Grl-1; Grl1; NR3C1
If an Invitrogen™ antibody doesn’t perform as described on our website or datasheet, we’ll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.