|Flow Cytometry (Flow)||1-2 ug/test|
|Gel Shift (GS)||Assay dependent|
|Immunocytochemistry (ICC)||Assay dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||5 µg/ml|
|Immunohistochemistry (PFA fixed) (IHC (PFA))||1/500|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||5 µg/ml|
|Western Blot (WB)||See 61 publications below|
|ChIP assay (ChIP)||See 11 publications below|
|Miscellaneous PubMed (MISC)||See 1 publications below|
|Immunohistochemistry (IHC)||See 7 publications below|
|Immunoprecipitation (IP)||See 20 publications below|
|Flow Cytometry (Flow)||See 3 publications below|
|Immunocytochemistry (ICC)||See 12 publications below|
|Gel Shift (GS)||See 5 publications below|
|ELISA (ELISA)||See 2 publications below|
|Tested Species reactivity||Guinea pig, Human, Mouse, Sheep, Rabbit, Rat, Yeast|
|Published species reactivity||Yeast , Rabbit , Rat , Non-human primate , Sheep , Human , Mouse , Xenopus|
|Host / Isotype||Mouse / IgG2|
|Immunogen||Partially purified rat GR.|
|Storage buffer||PBS, pH 7.2|
|Contains||0.05% sodium azide|
|Storage conditions||-20° C, Avoid Freeze/Thaw Cycles|
MA1-510 detects glucocorticoid receptor (GR) from human, mouse, rat, guinea pig, rabbit, sheep and yeast samples. This antibody does not react with primate, avian or amphibian GR.
MA1-510 has been successfully used in Western blot, immunofluorescence, immunocytochemistry, flow cytometry, immunohistochemistry, immunoprecipitation, and gel shift procedures. By Western blot, this antibody detects a 97 kDa protein representing GR in L929 cell extract. Immunocytochemical staining of GR in L929 cells with MA1-510 results in staining of both the cytoplasm and nucleus, even in the presence of hormone. Using enzymatic digestion analysis, MA1-510 reacts with the undigested 97 kDa GR, a 17 kDa DNA-binding trypsin fragment, and a 45 kDa steroid- and DNA-binding chymotrypsin fragment.
The MA1-510 immunogen is partially purified rat GR.
Reconstitute with 100ul distilled water.
Steroid receptors are ligand-dependent, intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. Glucocorticoids are a family of steroids necessary for the regulation of energy metabolism and the immune and inflammatory responses. These compounds exert their effect through their interaction with the glucocoticoid receptor (GR) and that complex's subsequent association with DNA. All normal mammalian tissues examined to date have been shown to contain glucocorticoid receptor.
The corresponding gene for the glucocorticoid receptor is NR3C1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: GCCR; GCR; glucocorticoid nuclear receptor variant 1; Glucocorticoid receptor; glucocorticoid receptor 1; glucocorticoid receptor alpha; Glucocorticoid receptor lymphocyte; GR; GRL; Grl1; NR3C1; Nuclear receptor subfamily 3 group C member 1; nuclear receptor subfamily 3, group C, member 1 (glucocorticoid receptor)
Gene Aliases: GCCR; GCR; GCRST; GR; GRL; Grl-1; Grl1; NR3C1
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