Immunofluorescence study using the anti-hGR monoclonal antibody. COS-7 cells transiently overexpressing human GR were labeled with the monoclonal anti-hGR antibody followed by biotinylated second antibody and peroxidase-labeled avidin. The monoclonal antibody directed against GR (clone: m2F8) is used at 2.5 µg⁄ml.
|Tested species reactivity||Human|
|Host / Isotype||Mouse / IgG|
|Immunogen||Raised against the aa 304-428 of human glucocorticoid receptor.|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|ChIP assay (ChIP)||5 ug|
|ELISA (ELISA)||0.5 ug/ml|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (IHC)||2.5 ug/ml|
|Western Blot (WB)||1.0 ug/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
NR3C1 is a receptor for glucocorticoids that can act as both a transcription factor and as a regulator of other transcription factors. This protein can also be found in heteromeric cytoplasmic complexes along with heat shock factors and immunophilins. The protein is typically found in the cytoplasm until it binds a ligand, which induces transport into the nucleus. Mutations in this gene are a cause of glucocorticoid resistance, or cortisol, resistance.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.