Western blot detection of Glucose Oxidase in (lane 1) 1µg Glucose Oxidase Reduced, (lane 2) 0.25µg Glucose Oxidase Reduced, (lane 3) 1µg Glucose Oxidase Non-Reduced, (lane 4) 0.25µg Glucose Oxidase, Non-Reduced using a Glucose Oxidase polyclonal antibody (Product # PA1-26994) at a dilution of 1:5000 following incubation overnight at 4°C and detected using a Dylight 649 conjugated Donkey anti Goat secondary antibody at a 1:10,000 dilution for 1.5 hrs at room temperature. Predicted/Observed size: 65.6 kDa, 90 kDa for Glucose Oxidase.
|Tested species reactivity||Fungi|
|Host / Isotype||Goat / IgG|
|Immunogen||Glucose Oxidase. [Apergillus niger]|
|Storage buffer||whole serum|
|Contains||0.05% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay Dependent|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-26994 detects Glucose Oxidase from Aspergillus niger samples.
PA1-26994 has been successfully used in immunohistochemistry (paraffin, frozen), ELISA, and Western blot procedures. This antibody does not require heat mediated antigen retrieval before staining.
The PA1-26994 immunogen is Glucose Oxidase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.