|Tested species reactivity||Bovine, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues S(475) D K T P E E L F H P L G A D S Q V(492) of rat GLUT1.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||Assay Dependent|
|Immunofluorescence (IF)||Assay dependent|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-1063 detects GLUT1 in rat, mouse and bovine samples.
PA1-1063 has been successfully used in Western blot and immunofluorescence procedures. By Western blot, this antibody detects a
~42 kDa protein representing GLUT1 in rat, mouse and bovine brain samples.
The PA1-1063 immunogen is a synthetic peptide corresponding to residues S(475) D K T P E E L F H P L G A D S Q V(492) of rat GLUT1. This sequence is conserved across many species including mouse, bovine, human, porcine and ovine. This peptide (Cat. # PEP-289) is available for use in neutralization and control experiments.
Glucose transporters are integral membrane glycoproteins involved in transporting glucose into most cells. Seven types of glucose transport carrier proteins designated as Glut 1 to 7 facilitate glucose transport across the cell membrane. Molecular cloning of glucose transporters have identified a family of closely related genes that encodes at least 7 proteins exhibiting high degree of amino acid homology (45% to 65%) all in the molecular weight range of 40 to 60 kDa.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.