|Tested species reactivity||Human, Rat|
|Published species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide to the C-terminal region of human GLUT1. The peptide sequence is considered commercially sensitive.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||Assay dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:200|
|Western Blot (WB)||0.5 µg/ml for 2 hrs|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-21041 detects glucose transporter (GLUT1) in human and rat samples.
PA1-21041 has been successfully used in immunofluorescence, immunohistochemistry (paraffin) and Western blot procedures. Staining of formalin fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 minutes.
PA1-21041 immunogen corresponds to a synthetic peptide to the C-terminal region of human GLUT1. The peptide sequence is considered commercially sensitive.
Glucose transporters are integral membrane glycoproteins involved in transporting glucose into most cells. Seven types of glucose transport carrier proteins designated as Glut 1 to 7 facilitate glucose transport across the cell membrane. Molecular cloning of glucose transporters have identified a family of closely related genes that encodes at least 7 proteins exhibiting high degree of amino acid homology (45% to 65%) all in the molecular weight range of 40 to 60 kDa. Individual members of the Glut family have predicted secondary structure characteristics of 12 membrane spanning domains of the transport carriers. Majority of the difference in sequence homology in Glut proteins occur at 4 hydrophilic domains that may play a role in distinct tissue specific pattern of expression and targeting. All Glut proteins are glycosylated at or near the C-terminus and are present on either cell surface or intracellular sites. Some transporters exhibit dynamic trafficing between intracellular storage sites and plasma membranes in response to various stimuli. In some tissues Glut proteins are assymmetrically distributed between apical and basolateral membranes as in blood brain barrier and blood tetis barriers.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Development of passive CLARITY and immunofluorescent labelling of multiple proteins in human cerebellum: understanding mechanisms of neurodegeneration in mitochondrial disease.
PA1-21041 was used in immunohistochemistry to use CLARITY to study mechanisms of neurodegeneration that occur in mitochondrial disease
|Phillips J,Laude A,Lightowlers R,Morris CM,Turnbull DM,Lax NZ||Scientific reports (6:null)||2016|
Brain microvascular accumulation and distribution of the NOTCH3 ectodomain and granular osmiophilic material in CADASIL.
PA1-21041 was used in immunohistochemistry to study the microvascular distribution of the Notch3 ectodomain and granular osmiophilic material in the brains of patients with CADASIL
|Yamamoto Y,Craggs LJ,Watanabe A,Booth T,Attems J,Low RW,Oakley AE,Kalaria RN||Journal of neuropathology and experimental neurology (72:416)||2013|
Adenomatoid tumors of the female and male genital tract. A comparative clinicopathologic and immunohistochemical analysis of 47 cases emphasizing their site-specific morphologic diversity.
PA1-21041 was used in immunohistochemistry to characterize human genital tract tumor
|Wachter DL,Wünsch PH,Hartmann A,Agaimy A||Virchows Archiv : an international journal of pathology (458:593)||2011|
Examination of glucose transporter-1, transforming growth factor-ß and neuroglobin immunoreactivity in the orbitofrontal cortex in late-life depression.
PA1-21041 was used in immunohistochemistry to identify proteins involved in hypoxic/ischemic signaling in orbitofrontal cortex
|Khundakar A,Morris C,Slade J,Thomas AJ||Psychiatry and clinical neurosciences (65:158)||2011|
Immunolocalization of platelet-derived growth factor receptor-ß (PDGFR-ß) and pericytes in cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL).
PA1-21041 was used in western blot to determine the pericyte distribution in relation to N3ECD deposits in cerebral microvessels in patients with cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy
|Craggs LJ,Fenwick R,Oakley AE,Ihara M,Kalaria RN||Neuropathology and applied neurobiology (41:557)||2015|