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|Tested species reactivity||Rat|
|Published species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues N(556) G K S A D C S V E E E P W K R E K(573) or rat GLT-1.|
|Storage buffer||whole serum diluted in PBS|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA3-040A detects glutamate transporter 1 (GLT-1) from rat tissues.
PA3-040A has been successfully used in Western blot and immunoprecipitation procedures. By Western blot, this antibody detects a ~61 kDa band representing GLT-1 from rat brain extract. This antibody may also detect one or more non-specific bands below 50 kDa that have not been identified.
The PA3-040A immunogen is a synthetic peptide corresponding to residues N(556) G K S A D C S V E E E P W K R E K(573) or rat GLT-1. While GLT-1, GLAST and EAAC1 share approximately 50% sequence homology, the sequence used to generate this product is unique to GLT-1.
Glutamate transporters (GluT) function to remove L-glutamate (Glu), the primary excitatory neurotransmitter in the mammalian central nervous system (CNS), from the synaptic cleft. By clearing the synapse in this manner, Glu can be recycled for later use, the proper diffusion gradient can be maintained and excitotoxicity can be prevented.
There have been reports of at least three different members of a GluT multigene family; GLT-1, GLAST and EAAC1. mRNA has been identified in brain for each of these proteins, while EAAC1 mRNA has also been shown to be transcribed in other tissues. GLT-1 is translated in numerous CNS regions including cerebellum, thalamus, hippocampus, midbrain and spinal cord. Unmodified GLT-1 has a calculated mass of 62 kDa protein (~75 kDa after post-translational modifications) in rat brain homogenates. A 51 kDa species resulting from an alternate translation initiation site has also been detected in rat brain homogenates.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Purkinje neuron Ca2+ influx reduction rescues ataxia in SCA28 model.
PA3-040A was used in western blot to explore the role of calcium handling in SCA28 pathogenesis
|Maltecca F,Baseggio E,Consolato F,Mazza D,Podini P,Young SM,Drago I,Bahr BA,Puliti A,Codazzi F,Quattrini A,Casari G||The Journal of clinical investigation (125:263)||2015|
Sumoylation of the astroglial glutamate transporter EAAT2 governs its intracellular compartmentalization.
PA3-040A was used in immunoprecipitation to study the role of sumoylation in determining the subcellular compartmentalization of the EAAT2 astrogial glutamate transporter
|Foran E,Rosenblum L,Bogush A,Pasinelli P,Trotti D||Glia (62:1241)||2014|
excitatory amino acid transporter 2; high affinity glucose transporter; sodium-dependent glutamate/aspartate transporter 2
Eaat2; Glt; Glt-1; Glt1; Slc1a2