Immunohistochemical analysis of Glutamic Acid Decarboxylase 65 using anti-Glutamic Acid Decarboxylase 65 Polyclonal Antibody (PA5-32430) in Pancreas Cancer Tissue. The recommened dilution for this antibody in immunohistochemistry applications is 1:50.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide from C-terminus of human GAD65.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is pancreas tissue.
Adults express two forms of glutamate decarboxylase (GAD), GAD65 and GAD67, which are encoded by different independently regulated genes. Both are expressed in most GABA containing neurons. GAD65 appears to be targeted to the membrane and nerve endings; while GAD67 is more widely distributed throughout the cell. GAD65 preferentially synthesizes GABA for vesicular release and GAD67 preferentially synthesizes cytoplasmic GABA. GAD65 is predominantly expressed in pancreatic beta cells and autoantibodies to GAD65 are strongly associated with Insulin-dependent diabetes mellitus (IMDM).
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