|Tested species reactivity||Human, Rat|
|Host / Isotype||Sheep / IgG|
|Immunogen||Synthetic peptide corresponding to residues R(175) R Y S R R F Q T I D I E P D I E A L L(194) of human glutathione peroxidase 1 ( GPx-1).|
|Storage buffer||whole serum|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1:2000-1:20,000|
|Immunohistochemistry (Paraffin) (IHC (P))||1:2000-1:20,000|
|Western Blot (WB)||1:10,000-1:100,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-18279 detects Glutathione Peroxidase 1 from human and rat samples.
PA1-18279 has been successfully used in immunohistochemistry (paraffin and frozen), ELISA and Western blot procedures.
The PA1-18279 immunogen is a synthetic peptide corresponding to residues R(175) R Y S R R F Q T I D I E P D I E A L L(194) of human glutathione peroxidase 1 ( GPx-1), conjugated to diphtheria toxin.
After reconstitution keep aliquots at -20°C for a higher stability, and at 4°C with an appropriate antibacterial agent. Avoid freeze/thaw cycles. Glycerol (1:1) may be added for an additional stability.
Reconstitute with 100 ul of distilled water.
This gene encodes a member of the glutathione peroxidase family. Glutathione peroxidase functions in the detoxification of hydrogen peroxide, and is one of the most important antioxidant enzymes in humans. This protein is one of only a few proteins known in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by UGA, that normally functions as a translation termination codon. In addition, this protein is characterized in a polyalanine sequence polymorphism in the N-terminal region, which includes three alleles with five, six or seven alanine (ALA) repeats in this sequence. The allele with five ALA repeats is significantly associated with breast cancer risk. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.