|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to the glycine-binding region residues S(140) Q V D L L A R L L R D L G(153) C of rat GBP.|
|Storage buffer||whole serum|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
PA3-100 detects the glycine-binding protein.
PA3-100 has been used successfully in Western blot, ELISA, immunohistochemistry, immunocytochemistry, and immunoprecipitation procedures. In Western blot analysis of rat brain synaptic membranes this antibody detects a ~60 kDa protein representing glycine-binding protein.
The PA3-100 immunogen is a synthetic peptide corresponding to the glycine-binding region residues S(140) Q V D L L A R L L R D L G(153) C of rat GBP.
Ionotropic glutamate receptors are oligomeric ion channel proteins, which mediate fast neurotransmission and excitatory synapses in the mammalian central nervous system. There are three distinct subfamilies, of which NMDA receptors have received particular attention because they are implicated in both synaptic plasticity and the pathogenesis of acute and chronic neurodegenerative disorders. A unique feature of the NMDA receptors is their requirement for both glutamate and the co-agonist glycine for efficient gating. Glycine increases the affinity of the receptor for the endogenous agonist glutamate. The glycine binding site is found on the NR1 subunit of the NMDA receptor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
A synaptic membrane glycine-, glutamate- and thienylcyclohexylpiperidine-binding protein: isolation and immunochemical characterization.
PA3-100 was used in western blot to purify and characterize a glycine, glutamate and TCP-binding protein from brain synaptic membranes
|Babcock KK,Chen X,Eggeman KT,Kumar KN,Decedue CJ,Michaelis EK||Neurochemistry international (29:507)||1996|